Recombinant Protein G (HRP) (ab7460)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% n/a
- Suitable for: WB, Dot blot, ELISA, IHC-P
製品の詳細
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製品名
Recombinant Protein G (HRP)
Protein G タンパク質・ペプチド 製品一覧 -
精製度
> 98 % n/a.
Protein G Peroxidase was prepared from chromatographically pure recombinant Protein G. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Streptococcus -
配列
L DKYGVSDYHK NLINNAKTVE GVKDLQAQVV ESAKKARISE ATDGLSDFLK SQTPAEDTVK SIELAEAKVL ANRELDKYGV SDYYKNLINN AKTVEGVKAL IDEILAALPK TDTYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLK -
予測される分子量
63 kDa -
領域
190 to 384
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標識
HRP
特性
Our Abpromise guarantee covers the use of ab7460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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製品の状態
Lyophilized -
備考
No reaction was observed against anti-Protein A. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
再構成Reconstitute product with 1ml of deionized water.
関連情報
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別名
- IgG binding protein G
- IgG-binding protein G
- Immunoglobulin G binding protein G [Precursor]
see all -
関連性
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. -
細胞内局在
Cell Wall and Secreted
画像
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Western blot - Recombinant Protein G (HRP) (ab7460)Image courtesy of an anonymous Abreview.Used for detection of primary antibody in WB after IP with the same antibody. In this case, IP was with goat anti HP1-alpha ab77256 on Protein G beads, and detection was with goat anti HP1-alpha ab77256, and Protein G HRP ab7460. Detection was very specific, and eliminated heavy and light chains of denatured antibodies.
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Dot Blot - Recombinant Protein G (HRP) (ab7460)Dot Blot analysis of ab7460 Protein G protein (HRP).
Load: 200ng of ab7460 followed by a 3-fold serial dilution
Antigens:
Row 1: Human IgG
Row 2: Rat IgG
Row 3: Dog IgG
Primary antibody: none
Secondary antibody: Protein G Peroxidase Conjugated at 1/1000 for 60 min at room temperature
Block: Blocking buffer for 60 min at room temperature
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (11)
ab7460 は 11 報の論文で使用されています。
- Lee J et al. Abnormal triaging of misfolded proteins by adult neuronal ceroid lipofuscinosis-associated DNAJC5/CSPα mutants causes lipofuscin accumulation. Autophagy 19:204-223 (2023). PubMed: 35506243
- Wu F et al. Acetylation-dependent coupling between G6PD activity and apoptotic signaling. Nat Commun 14:6208 (2023). PubMed: 37798264
- Ng L et al. Celastrol Downmodulates Alpha-Synuclein-Specific T Cell Responses by Mediating Antigen Trafficking in Dendritic Cells. Front Immunol 13:833515 (2022). PubMed: 35309340
- Huettner I et al. Cross-reactivity of glycan-reactive HIV-1 broadly neutralizing antibodies with parasite glycans. Cell Rep 38:110611 (2022). PubMed: 35354052
- Viard J et al. Chr21 protein-protein interactions: enrichment in proteins involved in intellectual disability, autism, and late-onset Alzheimer's disease. Life Sci Alliance 5:N/A (2022). PubMed: 35914814
