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AB52213

Recombinant Protein A + Protein G

Recombinant Protein A + Protein G

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(1 Publication)

Recombinant Protein A + Protein G is a Staphylococcus aureus subsp. aureus NCTC 8325 Fragment protein, expressed in Escherichia coli, with >98%, suitable for SDS-PAGE, AP.

別名を表示する

SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

1 Images
SDS-PAGE - Recombinant Protein A + Protein G (AB52213)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Protein A + Protein G (AB52213)

4-20% SDS-PAGE of Protein A/G : 10, 15 and 20 µg of Recombinant Protein A/G loaded in each lane under reducing conditions and stained with Coomassie Blue. Recombinant Protein A/G has a predicted MW of ∼ 59.7kDa.

Key facts

精製度

>98% SDS-PAGE

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

SDS-PAGE, AP

applications

生物活性

No

アクセッション番号

P02976

アニマルフリー

No

キャリアフリー

No

種

Staphylococcus aureus subsp. aureus NCTC 8325

再構成

Reconstitute at 5 mg/mL in water

バッファー組成

Constituents: 0.87% Sodium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "AP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Under optimal conditions, 1 mg protein A/G will bind approximately 5 mg human IgG. Optimal binding of Protein A/G to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0.</p>" } } }
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製品の詳細

This product is manufactured by BioVision, an Abcam company and was previously called 6502 Protein A/G. 6502-1 is the same size as the 1 mg size of ab52213.

This product is a genetically engineered fusion protein that combines the IgG binding profiles of both Protein A and Protein G. Protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. The 6x His-tag on the N-terminus can be used for affinity purification or for protein A/G detection using an anti-His-tag antibody. Non-specific binding regions, including cell wall binding regions, albumin binding regions and other non-specific binding regions have been eliminated from this fusion protein A/G, to ensure the maximum specific IgG binding.

Protein A+G binds to all IgG subclasses (from various mammalian species).

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配列情報

[{"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":"59.7 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P02976","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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製品の性状

製品の状態

Lyophilized

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一般的な情報

機能

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).

配列の類似性

Belongs to the immunoglobulin-binding protein SpA family.

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製品プロトコール

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ターゲットの情報

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
See full target information spa

追加のターゲット

spg,
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of chemical neuroanatomy 39:151-65 PubMed20043984

2010

The anatomy of the Göttingen minipig hypothalamus.

Applications

Unspecified application

Species

Unspecified reactive species

Kaare S Ettrup,Jens Christian Sørensen,Carsten R Bjarkam
View all publications
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組合せ製品

Reagents, Controls & Accessories

AB313996

Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (3 - 260 kDa) (AB313996)

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Proteins & Peptides

AB52953

Recombinant Staphylococcus aureus Protein A

Size Exclusion Chromatography - Recombinant Staphylococcus aureus Protein A (AB52953)

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Proteins & Peptides

AB7403

Streptavidin (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (AB7403)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
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Reagents, Controls & Accessories

AB119211

InstantBlue® Coomassie Protein Stain (ISB1L)

Western blot - InstantBlue® Coomassie Protein Stain (ISB1L) (AB119211)

  • 5
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Abcam product promise

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