Recombinant Protein A + Protein G (ab52213)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Protein A + Protein G -
生理活性
Protein A+G binds to all IgG subclasses (from various mammalian species). -
精製度
> 98 % SDS-PAGE. -
発現系
Escherichia coli -
タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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実際の分子量
60 kDa including tags -
タグ
His tag N-Terminus -
配列の追加情報
Protein A: P02976 Protein G: P19909
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関連製品
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Positive Controls
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Related Products
特性
Our Abpromise guarantee covers the use of ab52213 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Affinity Purification
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製品の状態
Lyophilized -
備考
This product is manufactured by BioVision, an Abcam company and was previously called 6502 Protein A/G. 6502-1 is the same size as the 1 mg size of ab52213.
This product is a genetically engineered fusion protein that combines the IgG binding profiles of both Protein A and Protein G. Protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. The 6x His-tag on the N-terminus can be used for affinity purification or for protein A/G detection using an anti-His-tag antibody. Non-specific binding regions, including cell wall binding regions, albumin binding regions and other non-specific binding regions have been eliminated from this fusion protein A/G, to ensure the maximum specific IgG binding.
Protein A+G binds to all IgG subclasses (from various mammalian species).
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
再構成Reconstitute in water to a concentration of 5 mg/ml.
関連情報
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細胞内局在
Protein A: Secreted; cell wall. Protein G: Cell Wall and Secreted
画像
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SDS-PAGE - Recombinant Protein A + Protein G (ab52213)
4-20% SDS-PAGE of Protein A/G: 10, 15 and 20 µg of Recombinant Protein A/G loaded in each lane under reducing conditions and stained with Coomassie Blue. Recombinant Protein A/G has a predicted MW of ∼ 59.7kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab52213 は 1 報の論文で使用されています。
- Ettrup KS et al. The anatomy of the Göttingen minipig hypothalamus. J Chem Neuroanat 39:151-65 (2010). PubMed: 20043984
