Recombinant Picornain 3C protein (ab157275)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant Picornain 3C protein -
生理活性
Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as It has been demonstrated that the enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37?, even retaining robust activity at 4?. Thus, cleavage can be performed at low temperature to enhance the stability of the target protein.SpecificityThe enzyme recognizes the cleavage site: Leu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro.
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精製度
> 90 % SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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配列
GPNTEFALSLLRKNIMTITTSKGEFTGLGIHDRVCVIPTHAQPGDDVLVN GQKIRVKDKYKLVDPENINLELTVLTLDRNEKFRDIRGFISEDLEGVDAT LVVHSNNFTNTILEVGPVTMAGLINLSSTPTNRMIRYDYATKTGQCGGVL CATGKIFGIHVGGNGRQGFSAQLKKQYFVEKQ -
予測される分子量
22 kDa -
領域
1 to 182
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特性
Our Abpromise guarantee covers the use of ab157275 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as It has been demonstrated that the enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37°C, even retaining robust activity at 4°C. Thus, cleavage can be performed at low temperature to enhance the stability of the target protein. The catalytic activity is insensitive to organic solvents (up to 10%); however, it can be strongly stimulated by high concentration of anions such as sulfate.
The high specificity of ab157275 makes it an ideal tool for cleaving fusion proteins at definite cleavage sites. The enzyme recognizes the cleavage site: Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. >1 Units/µg. One unit will cleave >95% of 100 µg control fusion protein in 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 at 4°C for 16 h. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.4
Constituents: 0.61% Tris, 0.05% Tween, 0.03% EDTA, 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with 50% glycerol to a concentration of 1 mg/ml.
関連情報
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別名
- 3C
- P3C
- Protease 3C
画像
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Functional Studies - Recombinant Picornain 3C protein (ab157275)A 52 kDa Fc-Fusion protein (C) at 1/mg/ml is incubated with ab157275 at the ratio of (1) 1:50 (2) 1:100 (3) 1:200 (4) 1:400 (5) 1:800 (6) 1:1000 (w/w) in a buffer of 50 mM TRIS-HCl, 150mM NaCl, 1mM EDTA, 1mM dithiothreitol, pH 7.0 at 4 degC for 16 hours.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab157275 は論文での使用が確認できていません。
