Recombinant Mycobacterium tuberculosis Ag85B protein (ab83471)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
製品の詳細
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製品名
Recombinant Mycobacterium tuberculosis Ag85B protein -
精製度
> 90 % SDS-PAGE.
Endotoxin Tested -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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配列
MTDVSRKIRA WGRRLMIGTA AAVVLPGLVG LAGGAATAGA FSRPGLPVEY LQVPSPSMGR DIKVQFQSGG NNSPAVYLLD GLRAQDDYNG WDINTPAFEW YYQSGLSIVM PVGGQSSFYS DWYSPACGKA GCQTYKWETF LTSELPQWLS ANRAVKPTGS AAIGLSMAGS SAMILAAYHP QQFIYAGSLS ALLDPSQGMG PSLIGLAMGD AGGYKAADMW GPSSDPAWER NDPTQQIPKL VANNTRLWVY CGNGTPNELG GANIPAEFLE NFVRSSNLKF QDAYNAAGGH NAVFNFPPNG THSWEYWGAQ LNAMKGDLQS SLGAG -
予測される分子量
30 kDa -
タグ
His tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab83471 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Store at -80°C.
pH: 7.50
Constituents: 0.24% Monobasic dihydrogen sodium phosphate, 0.284% Dibasic monohydrogen sodium phosphate, 0.328% Sodium phosphate, 10% Glycerol, 2.9% Sodium chloride -
再構成ab83471 should be reconstituted in apirogenic water or PBS and has to be used immediately
関連情報
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別名
- 30 kDa extracellular protein
- Antigen 85 complex B
- Mycobacterium
- Mycolyl transferase 85B
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関連性
Antigen 85B is the most abundant protein expressed by Mycobacterium tuberculosis (about one quarter). It is a myocolyc transferase in the myc pathway and catalyses - like Ag85A and Ag85C - the transfer of the fatty acid mycolate from one trehalose monomycolate to another, resulting in trehalose dimycolate and free trehalose and helping build the cell wall.
画像
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SDS-PAGE - Recombinant Mycobacterium tuberculosis Ag85B protein (ab83471)
SDS Page 12% polyacrylamide gel with 5µg ab83471 stained with Coomassie Blue in lane 2 compared with the molecular marker in lane 1.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab83471 は 1 報の論文で使用されています。
- Luo L et al. Triamcinolone acetonide induces the autophagy of Ag85B-treated WI-38 cells via SIRT1/FOXO3 pathway. Allergol Immunopathol (Madr) 51:27-35 (2023). PubMed: 36916085