Recombinant mouse VEGFA protein (Active) (ab185265)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant mouse VEGFA protein (Active)
VEGFA タンパク質・ペプチド 製品一覧 -
生理活性
Measured in a cell proliferation assay using Human umbilical vein endothelial cells ( HUVEC ). The ED50 for this effect is typically 3.5 ng/ml.
Measured by its binding ability in a functional ELISA. Immobilized ab185265 at 2 µg/mL (100 µL/well) can bind VEGFR1/R2-Fc with a linear range of 0.6-2.5 ng/mL.
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精製度
> 95 % SDS-PAGE.
>95% as determined by SEC-HPLC. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
予測される分子量
19 kDa -
領域
27 to 190 -
配列の追加情報
Mature form. Isoform VEGF-1 or VEGF 164. AAB22253.1
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab185265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 97% PBS, 3% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with sterile deionized water to a concentration of 200 µg/ml.
関連情報
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別名
- Folliculostellate cell-derived growth factor
- Glioma-derived endothelial cell mitogen
- MGC70609
see all -
機能
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
組織特異性
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
関連疾患
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
細胞内局在
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
画像
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SDS-PAGE - Recombinant mouse VEGFA protein (Active) (ab185265)
ab185265 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant mouse VEGFA protein (Active) (ab185265)The purity of ActiveMax® Mouse VEGF164 was greater than 95% as determined by SEC-HPLC. -
Functional Studies - Recombinant mouse VEGFA protein (Active) (ab185265)Immobilized ab185265 at 2 µg/mL (100 µL/well) can bind VEGFR1/R2-Fc with a linear range of 0.6-2.5 ng/mL.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab185265 は 3 報の論文で使用されています。
- Lu Y et al. Inhibition of FNDC1 suppresses gastric cancer progression by interfering with Gβγ-VEGFR2 complex formation. iScience 26:107534 (2023). PubMed: 37670789
- Zhang J et al. Irisin Attenuates Pathological Neovascularization in Oxygen-Induced Retinopathy Mice. Invest Ophthalmol Vis Sci 63:21 (2022). PubMed: 35737379
- Xue Y et al. Forskolin promotes vasculogenic mimicry and invasion via Notch-1-activated epithelial-to-mesenchymal transition in syncytiolization of trophoblast cells in choriocarcinoma. Int J Oncol 56:1129-1139 (2020). PubMed: 32319581
