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Recombinant mouse TRAP/CD40L protein (Soluble) (ab50160)

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Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

製品の詳細

  • 製品名

    Recombinant mouse TRAP/CD40L protein (Soluble)
    TRAP/CD40L タンパク質・ペプチド 製品一覧

  • 生理活性

    Determined by its ability to induce TNF-α and MIP-1α production by murine splenocytes. The expected ED50 for this effect is < 0.1 µg/ml.

  • 精製度

    > 98 % SDS-PAGE.
    Greater than 98% by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g). Lyophilized from 10mM Sodium Phosphate, pH 7.5 + 0.1 M Arginine.

  • 発現系

    Escherichia coli

  • アクセッション番号

    P27548

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Mouse

    • 配列

      MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL

    • 領域

      112 to 260

    • 配列の追加情報

      Full length TRAP/CD40L, soluble form

特性

Our Abpromise guarantee covers the use of ab50160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    SDS-PAGE

    Functional Studies

  • 製品の状態

    Lyophilized
  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 再構成
    For lot specific reconstitution information please contact our Scientific Support Team.

関連情報

  • 別名

    • CD 40L
    • CD154
    • CD40 antigen ligand
    • CD40 ligand
    • CD40 ligand, soluble form
    • CD40-L
    • CD40L
    • CD40L_HUMAN
    • CD40LG
    • gp39
    • hCD40L
    • HIGM1
    • IGM
    • IMD3
    • T B cell activating molecule
    • T BAM
    • T-cell antigen Gp39
    • TNF-related activation protein
    • TNFSF5
    • TrAP
    • Tumor necrosis factor (ligand) superfamily member 5
    • Tumor necrosis factor ligand superfamily member 5
    see all

  • 機能

    Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
    Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.

  • 組織特異性

    Specifically expressed on activated CD4+ T-lymphocytes.

  • 関連疾患

    Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.

  • 配列類似性

    Belongs to the tumor necrosis factor family.

  • 翻訳後修飾

    The soluble form derives from the membrane form by proteolytic processing.
    N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
    Not O-glycosylated.

  • 細胞内局在

    Secreted and Cell membrane.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab50160 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab50160 は論文での使用が確認できていません。

レビューと Q&A

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Question

I would like to use ab50160 to activate cell, what concentration should I treat cell?

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Answer

Thank you for your enquiry. The bioassay for our Murine CD40 Ligand was determined using murine splenocytes to induce TNF-alpha and MIP-1 alpha. The ED50 for this is [0.1ug/ml] Please note that cytokines will react differently on different cell lines and conditions. There is also a reference on our website, where this cytokine has been used for dendritic cell maturation and activation. Please follow the hyperlink below for further details: http://www.jimmunol.org/cgi/content/full/174/5/2720 Please see the paragraph below referring to the method used: For dendritic cell maturation and activation, cells were cultured at 1 x 10^6/ml in 24-well plates, and stimulated with 500 ng/ml CD40L and 1 µg/ml LPS for 48 h. I hope this information has been of help, please do not hesitate to contact me should you have any additional questions.

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