Recombinant Mouse TIMP1 Protein (Active) (ab291058)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, MS
製品の詳細
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製品名
Recombinant Mouse TIMP1 Protein (Active)
TIMP1 タンパク質・ペプチド 製品一覧 -
生理活性
Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGL[1]Dpa-AR-NH2).
ED50 is ≤ 2.8 ng/ml, corresponding to a specific activity of 3.6 x 105 units/mg.
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精製度
>= 95 % SDS-PAGE. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
由来
Recombinant -
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生物種
Mouse -
配列
CSCAPPHPQTAFCNSDLVIRAKFMGSPEINETTLYQRYKIKMTKMLKGFK AVGNAADIRYAYTPVMESLCGYAHKSQNRSEEFLITGRLRNGNLHISACS FLVPWRTLSPAQQRAFSKTYSAGCGVCTVFPCLSIPCKLESDTHCLWTDQ VLVGSEDYQSRHFACLPRNPGLCTWRSLGAR -
予測される分子量
23 kDa -
領域
25 to 205 -
タグ
His tag C-Terminus
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特性
Our Abpromise guarantee covers the use of ab291058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Mass Spectrometry
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Store lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- Clgi
- Collagenase inhibitor
- Collagenase inhibitor, Human
see all -
機能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
配列類似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻訳後修飾
The activity of TIMP1 is dependent on the presence of disulfide bonds. -
細胞内局在
Secreted. - Information by UniProt
画像
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Biological Activity - Recombinant Mouse TIMP1 Protein (Active) (ab291058)
Fully active determined by its ability to inhibit human MMP2 cleavage of a chromogenic peptide substrate (Mca-PLGL[1]Dpa-AR-NH2).
ED50 is ≤ 2.8 ng/ml, corresponding to a specific activity of 3.6 x 105 units/mg.
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SDS-PAGE - Recombinant Mouse TIMP1 Protein (Active) (ab291058)SDS-PAGE analysis of ab291058 -
Mass Spectrometry - Recombinant Mouse TIMP1 Protein (Active) (ab291058)Mass determination by ESI-TOF.
Observed MW is 21063.19 Da (+/- 10 Da by ESI-TOF).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab291058 は論文での使用が確認できていません。
