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AB271760

Recombinant Mouse TIGIT protein (Biotin) (Fc tag N-Terminus + Avi tag N-Terminus)

Recombinant Mouse TIGIT protein (Biotin) (Fc tag N-Terminus + Avi tag N-Terminus)

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Recombinant Mouse TIGIT protein (Biotin) (Fc tag N-Terminus + Avi tag N-Terminus) is a Mouse Fragment protein, in the 26 to 143 aa range, expressed in HEK 293 cells, with >90%, suitable for WB, SDS-PAGE.

別名を表示する

Vstm3, Tigit, T-cell immunoreceptor with Ig and ITIM domains, V-set and transmembrane domain-containing protein 3

2 Images
Western blot - Recombinant Mouse TIGIT protein (Biotin) (Fc tag N-Terminus + Avi tag N-Terminus) (AB271760)
  • WB

Supplier Data

Western blot - Recombinant Mouse TIGIT protein (Biotin) (Fc tag N-Terminus + Avi tag N-Terminus) (AB271760)

All lanes:

Western blot - Recombinant Mouse TIGIT protein (Biotin) (Fc tag N-Terminus + Avi tag N-Terminus) (ab271760) at 5 µg

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SDS-PAGE - Recombinant Mouse TIGIT protein (Biotin) (Fc tag N-Terminus + Avi tag N-Terminus) (AB271760)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse TIGIT protein (Biotin) (Fc tag N-Terminus + Avi tag N-Terminus) (AB271760)

SDS-PAGE analysis of 5 μg ab271760.

This protein runs at a higher MW by SDS-PAGE due to glycosylation.

Key facts

精製度

>90% SDS-PAGE

発現系

HEK 293 cells

タグ

Fc tag N-Terminus Avi tag N-Terminus

アプリケーション

WB, SDS-PAGE

applications

生物活性

No

標識

Biotin

励起波長/蛍光波長
アクセッション番号

P86176

アニマルフリー

No

キャリアフリー

No

種

Mouse

バッファー組成

pH: 7.4 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.02% Potassium chloride, 0.02% Sodium phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This protein runs at a higher MW by SDS-PAGE due to glycosylation.</p>" } } }
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製品の詳細

Enzymatically biotin-labeled using Avi-tag™ technology
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配列情報

[{"sequence":"TAGTIDTKRNISAEEGGSVILQCHFSSDTAEVTQVDWKQQDQLLAIYSVDLGWHVASVFSDRVVPGPSLGLTFQSLTMNDTGEYFCTYHTYPGGIYKGRIFLKVQESSDDRNGLAQFQ","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":143,"aminoAcidStart":26,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P86176","tags":[{"tag":"Fc","terminus":"N-Terminus"},{"tag":"Avi","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
保管に関する情報
Avoid freeze / thaw cycle|Store in the dark
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

TIGIT also known as T cell immunoreceptor with Ig and ITIM domains is an immunoglobulin superfamily receptor. It weighs approximately 26 kDa. TIGIT commonly expresses on T cells especially on regulatory T cells (Tregs) and effector memory T cells. It also presents on natural killer (NK) cells. Among its alternate forms some notable ones also include the anti-TIGIT and biotinylated TIGIT antibodies used in experimental research to monitor immune responses.
Biological function summary

TIGIT acts as an inhibitory receptor modulating immune responses. It belongs to the CD28 family and binds to poliovirus receptor (PVR) and nectin-2 on antigen-presenting cells. This interaction inhibits T cell activation and proliferation and increases Treg cell activity maintaining immune homeostasis. TIGIT forms a complex with other inhibitory receptors like PD-1 2B4 and CD96 to further regulate immune functions.

Pathways

The interaction of TIGIT with its ligands significantly affects the immune checkpoint pathway. This involvement regulates T cell and NK cell activity. TIGIT is related to CD226 (DNAM-1) which competes for binding to its ligands influencing cellular signaling pathways. Through these pathways TIGIT signals through the downstream SHP-1 and SHP-2 proteins affecting immune cell signaling and response.

TIGIT has implications in cancer and autoimmune diseases. Its inhibitory function can allow tumor progression by dampening effective anti-tumor immunity. In cancer TIGIT’s activity often pairs with PD-1 both contributing to immune checkpoint blockade resistance necessary for tumor evasion. In autoimmune diseases increased TIGIT expression could result in reduced immune cell attack against self tissues showing its potential to modulate treatment strategies.
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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway. Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity.

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製品プロトコール

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ターゲットの情報

Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades. In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway. Acts also as a receptor for NECTIN4 to inhibit NK cell cytotoxicity.
See full target information Tigit
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