Recombinant mouse Thrombopoietin protein (ab170079)
Key features and details
- Expression system: Insect cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
-
製品名
Recombinant mouse Thrombopoietin protein
Thrombopoietin タンパク質・ペプチド 製品一覧 -
生理活性
ED50 < 5 ng/ml as determined in a cell proliferation assay using Human TF-1 erythroleukemia cells.
-
精製度
> 95 % SDS-PAGE.
ab170079 is purified by chromatography, sterile filtered and lyophilized. -
発現系
Insect cells -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Mouse -
配列
SPVAPACDPRLLNKLLRDSHLLHSRLSQCPDVDPLSIPVLLPAVDFSLGE WKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLL LGALQGLLGTQLPLQGRTTAHKDPNALFLSLQQLLRGKVRFLLLVEGPTL CVRRTLPTTAVPSSTSQLLTLNKFPNRTSGLLETNFSVTARTAGPGLLSR LQGFRVKITPGQLNQTSRSPVQISGYLNRTHGPVNGTHGLFAGTSLQTLE ASDISPGAFNKGSLAFNLQGGLPPSPSLAPDGHTPFPPSPALPTTHGSPP QLHPLFPDPSTTMPNSTAPHPVTMYPHPRNLSQET -
予測される分子量
66 kDa -
領域
22 to 356
-
特性
Our Abpromise guarantee covers the use of ab170079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Functional Studies
-
製品の状態
Lyophilized -
備考
Produced using non-baculovirus insect cells.
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Store at -80°C.
pH: 7.4
Constituent: 100% PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
再構成Add 0.5ml deionized water. Aliquot and freeze unused portion at -80°C.
関連情報
-
別名
- C mpl ligand
- C-mpl ligand
- Megakaryocyte colony stimulating factor
see all -
機能
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. -
関連疾患
Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. -
配列類似性
Belongs to the EPO/TPO family. -
ドメイン
Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal. -
細胞内局在
Secreted. - Information by UniProt
画像
-
SDS-PAGE - Recombinant mouse Thrombopoietin protein (ab170079)SDS-PAGE analysis of ab170079.
Lane 1: 3µg ab170079 Reduced
Lane 2: 3µg ab170079 Non-reduced
Lane 3: Molecular weight markers -
Functional Studies - Recombinant mouse Thrombopoietin protein (ab170079)Proliferation of Human TF-1 erythroleukemia cells by ab170079.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (1)
ab170079 は 1 報の論文で使用されています。
- Zhang Y et al. Chemically modified in-vitro-transcribed mRNA encoding thrombopoietin stimulates thrombopoiesis in mice. Mol Ther Nucleic Acids 29:657-671 (2022). PubMed: 36090760
