Recombinant Mouse S100 alpha protein (ab108117)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
製品の詳細
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製品名
Recombinant Mouse S100 alpha protein -
精製度
> 90 % SDS-PAGE.
ab108117 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA. -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
配列
MGSSHHHHHHSSGLVPRGSHMGSELESAMETLINVFHAHSGKEGDKYKLS KKELKDLLQTELSGFLDVQKDADAVDKVMKELDENGDGEVDFKEYVVLVA ALTVACNNFFWETS -
予測される分子量
13 kDa including tags -
領域
1 to 94 -
タグ
His tag N-Terminus
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab108117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Mass Spectrometry
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質量分析
MALDI-TOF -
製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
関連情報
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別名
- Bpb
- NEF
- Protein S100-A1
see all -
機能
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
組織特異性
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
配列類似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
細胞内局在
Cytoplasm. - Information by UniProt
画像
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SDS-PAGE - Recombinant Mouse S100 alpha protein (ab108117)15% SDS-PAGE showing ab108117 at approximately 12.6kDa (3µg).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab108117 は論文での使用が確認できていません。