AB78590

Recombinant mouse PDGF AA protein

Name: Recombinant mouse PDGF AA protein (ab78590) | Abcam
Brand: Abcam Limited
SKU: AB78590
Price: 71000 JPY
Availability: InStock

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Recombinant mouse PDGF AA protein is a Mouse Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

別名を表示する

Platelet-derived growth factor subunit A, PDGF subunit A, PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, Pdgfa

2 Images

Functional Studies - Recombinant mouse PDGF AA protein (AB78590)

FuncS

Unknown

Functional Studies - Recombinant mouse PDGF AA protein (AB78590)

ab78590 used in Functional Studies.

SDS-PAGE - Recombinant mouse PDGF AA protein (AB78590)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant mouse PDGF AA protein (AB78590)

SDS PAGE analysis of ab78590 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

Key facts

精製度

>95% SDS-PAGE

発現系

Escherichia coli

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED₅₀ for this effect is 8-10 ng/ml.

アクセッション番号

P20033

アニマルフリー

キャリアフリー

種

Mouse

再構成

Reconstitute in water

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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製品の詳細

It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.

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配列情報

[{"sequence":"MSIEEAVPAVCKTRTVIYEIPRSQVDPTSANFLIWPPCVEVKRCTGCCNTSSVKCQPSRVHHRSVKVAKVEYVRKKPKLKEVQVRLEEHLECACATSNLNPDHREEETGRRRESGKNRKRKRLKPT","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P20033","tags":[]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Platelet-derived growth factor AA or PDGF-AA is a member of the PDGF protein family which is involved in various cellular processes. The PDGF-AA protein has a molecular mass of approximately 24 kDa. It is expressed in a wide range of tissues including fibroblasts smooth muscle cells and neurons. The protein is considered a homodimer composed of two A chains and it plays an important role in cell signaling by binding to PDGF receptors on the surface of target cells.

Biological function summary

PDGF-AA influences cell proliferation migration and differentiation. It acts as a mitogen especially for mesenchymal cells such as fibroblasts and smooth muscle cells. PDGF-AA is often part of a signaling complex where it activates PDGF receptor-alpha (PDGFR-α) leading to autophosphorylation and subsequent activation of downstream effectors. This strengthens tissue development and wound healing processes.

Pathways

PDGF-AA integrates into critical signaling pathways like the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell growth and survival. PDGF-AA binds specifically to PDGFR-α which shares importance with other PDGF isoforms such as PDGF-BB in orchestrating cellular responses through similar pathways thereby ensuring precise physiological outcomes.

PDGF-AA is implicated in conditions like cancer and fibrosis. Aberrant signaling involving PDGF-AA can lead to unchecked cell proliferation contributing to tumorigenesis. Similarly PDGF-AA is connected to fibrotic diseases where excessive tissue scarring occurs due to overactive fibroblast stimulation. PDGF-BB another member of the PDGF protein family often gets studied alongside PDGF-AA for their combined roles in these pathological states.

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製品の性状

製品の状態

Lyophilized

補足情報

Purity Greater than 97% as determined by reducing and non-reducing SDS-PAGE, and by analytical HPLC.This product was sterile filtered.

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一般的な情報

機能

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB.

配列の類似性

Belongs to the PDGF/VEGF growth factor family.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Pdgfa

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Abcam product promise

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Recombinant mouse PDGF AA protein