Recombinant mouse PAK3 protein (ab61414)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
-
製品名
Recombinant mouse PAK3 protein -
生理活性
Specific activity: 259 nmol/min/mg.
-
精製度
> 75 % Densitometry.
Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Mouse
-
関連製品
-
Related Products
-
Substrate reagent
特性
Our Abpromise guarantee covers the use of ab61414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
-
製品の状態
Liquid -
備考
ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- Beta PAK
- bPAK
- CDKN1A
see all -
関連性
PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. PAK3 forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. It may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. A point mutation in this gene has been linked to nonsyndromic X-linked mental retardation. -
細胞内局在
Cytoplasmic
画像
-
Functional Studies - Recombinant mouse PAK3 protein (ab61414)The specific activity of PAK3 (ab61414) was determined to be 54.2 nmol/min/mg as per activity assay protocol and was equivalent to 270 nmol/min/mg as per radiometric assay
-
SDS-PAGE - Recombinant mouse PAK3 protein (ab61414)SDS PAGE analysis of ab61414 -
SDS-PAGE - Recombinant mouse PAK3 protein (ab61414)SDS PAGE analysis of ab61414 -
Functional Studies - Recombinant mouse PAK3 protein (ab61414)Sample Kinase Activity Plot.
-
SDS-PAGE - Recombinant mouse PAK3 protein (ab61414)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab61414 は論文での使用が確認できていません。