Recombinant mouse M-CSF protein (ab129146)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant mouse M-CSF protein
M-CSF タンパク質・ペプチド 製品一覧 -
生理活性
Method of Determination: NFS-60 cell proliferation
Acception Criteria: ED50 ≤10 ng/mL(≥ 1.0 x 10^5 units/mg)
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精製度
>= 95 % SDS-PAGE.
Purified using standard chromatographical techniques. -
エンドトキシン・レベル
<=1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
配列
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKPMKEV SEHCSHMIGN GHLKVLQQLI DSQMETSCQI AFEFVDQEQL DDPVCYLKKA FFLVQDIIDE TMRFKDNTPN ANATERLQEL SNNLNSCFTK DYEEQNKACV RTFHETPLQL LEKIKNFFNE TKNLLEKDWN IFTKNCNNSF AKCSSRDVVT KP -
予測される分子量
36 kDa -
領域
33 to 187
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特性
Our Abpromise guarantee covers the use of ab129146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
備考
Endotoxin levels determined by kinetic LAL (50% Confidence).
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.16% Sodium phosphate, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Sterile water at 0.1 mg/mL Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
関連情報
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別名
- Colony stimulating factor 1
- Colony stimulating factor 1 (macrophage)
- Colony stimulating factor macrophage specific
see all -
機能
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. -
翻訳後修飾
Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated. -
細胞内局在
Cell membrane and Secreted > extracellular space. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab129146 は 5 報の論文で使用されています。
- Bresque M et al. SIRT6 stabilization and cytoplasmic localization in macrophages regulates acute and chronic inflammation in mice. J Biol Chem 298:101711 (2022). PubMed: 35150745
- Gao Z et al. Nociceptor Neurons are Involved in the Host Response to Escherichia coli Urinary Tract Infections. J Inflamm Res 15:3337-3353 (2022). PubMed: 35702548
- Huang C et al. Hepatocyte growth factor overexpression promotes osteoclastogenesis and exacerbates bone loss in CIA mice. J Orthop Translat 27:9-16 (2021). PubMed: 33344167
- Wang R et al. Urinary Trypsin Inhibitor Protects Tight Junctions of Septic Pulmonary Capillary Endothelial Cells by Regulating the Functions of Macrophages. J Inflamm Res 14:1973-1989 (2021). PubMed: 34045879
- Zheng Y et al. Gentiana scabra Restrains Hepatic Pro-Inflammatory Macrophages to Ameliorate Non-Alcoholic Fatty Liver Disease. Front Pharmacol 12:816032 (2021). PubMed: 35115947