Recombinant Mouse LIF protein (Active) (ab288791)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, MS, Functional Studies
製品の詳細
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製品名
Recombinant Mouse LIF protein (Active)
LIF タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependent inhibition of M1 cells.
ED50 is ≤ 0.22 ng/ml, corresponding to a specific activity of 4.5 x 106 units/mg.
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精製度
>= 95 % SDS-PAGE. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Mouse -
配列
PLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEP FPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQ KVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHS DKEAFQRKKLGCQLLGTYKQVISVVVQAF -
予測される分子量
22 kDa -
領域
25 to 203 -
配列の追加情報
N-terminal glycine
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特性
Our Abpromise guarantee covers the use of ab288791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Mass Spectrometry
Functional Studies
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphateThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
関連情報
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別名
- CDF
- Cholinergic Differentiation Factor
- D factor
see all -
機能
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. -
配列類似性
Belongs to the LIF/OSM family. -
細胞内局在
Secreted. - Information by UniProt
画像
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Functional Studies - Recombinant Mouse LIF protein (Active) (ab288791)
Fully biologically active determined by the dose dependent inhibition of M1 cells.
ED50 is ≤ 0.22 ng/ml, corresponding to a specific activity of 4.5 x 106 units/mg.
Cell based assay testing is performed on the first lot of the protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3443909-2.
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SDS-PAGE - Recombinant Mouse LIF protein (Active) (ab288791)SDS-PAGE analysis of ab288791 -
Mass Spectrometry - Recombinant Mouse LIF protein (Active) (ab288791)Mass determination by ESI-TOF.
Predicted MW is 19838 Da. (+/- 10 Da by ESI-TOF). Observed MW is 19841.58 Da.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab288791 は論文での使用が確認できていません。
