Recombinant mouse Jagged1 protein (Fc Chimera Active) (ab109346)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.010 Eu/µg
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies, Inhibition Assay
製品の詳細
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製品名
Recombinant mouse Jagged1 protein (Fc Chimera Active)
Jagged1 タンパク質・ペプチド 製品一覧 -
生理活性
Inhibits adipogenesis in NIH/3T3 cells.
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精製度
> 90 % SDS-PAGE.
ab109346 is lyophilised from a 0.2 µm filtered solution. Purified using affinity chromatography. -
エンドトキシン・レベル
< 0.010 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
予測される分子量
150 kDa including tags -
領域
1 to 1067 -
タグ
Fc tag C-Terminus -
配列の追加情報
Signal peptide and extracellular domain of mouse Jagged-1 (aa 1-1067) are fused at the C-terminus to the Fc portion of human IgG.
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特性
Our Abpromise guarantee covers the use of ab109346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
Inhibition Assay
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituent: PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成For 10µg size: reconstitute with 100µl sterile water. For 50µg size: reconstitute with 50µl sterile water
関連情報
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別名
- AGS
- AHD
- AWS
see all -
機能
Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro). -
組織特異性
Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells. -
関連疾患
Defects in JAG1 are the cause of Alagille syndrome type 1 (ALGS1) [MIM:118450]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
Defects in JAG1 are a cause of tetralogy of Fallot (TOF) [MIM:187500]. TOF is a congenital heart anomaly which consists of pulmonary stenosis, ventricular septal defect, dextroposition of the aorta (aorta is on the right side instead of the left) and hypertrophy of the right ventricle. This condition results in a blue baby at birth due to inadequate oxygenation. Surgical correction is emergent. -
配列類似性
Contains 1 DSL domain.
Contains 15 EGF-like domains. -
発生段階
Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube. -
細胞内局在
Membrane. - Information by UniProt
画像
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Inhibition Assay - Recombinant mouse Jagged1 protein (Fc Chimera Active) (ab109346)
Differentiation of 3T3-L1 cells was performed by first growing cells to confluence (day 2) and then shifting them to differentiation medium after 48 h (day 0). The differentiation medium contained 1 μM dexamethasone, 0.5 mM IBMX, and 15μg/ml of insulin. Differentiation medium lacking dexamethasone and IBMX and addition of mouse TNF-alpha (20mg/ml) were both used as a control. As a negative control hCD137-Fc was used. Medium was changed to growth medium containing only insulin (15μg/ml) after 48 h (day 2). Staining with Oil Red O was typically performed on day 7. Notch signaling was generated by immobilizing recombinant mouse Jagged-1-Fc on plates (Jagged-1 was coated on 12-well plate at 5µg/ml (1µg/ml should also work) overnight at 4°C). These plates (with or without Notch ligand) were then used to grow 3T3-L1 cells.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab109346 は 5 報の論文で使用されています。
- Huang D et al. Dlk1 maintains adult mice long-term HSCs by activating Notch signaling to restrict mitochondrial metabolism. Exp Hematol Oncol 12:11 (2023). PubMed: 36653853
- Yin Y et al. Atonal homolog 7 (ATOH7) confers neuroprotection for photoreceptor cells in glaucoma via inhibition of the notch pathway. J Neurochem 166:847-861 (2023). PubMed: 37526008
- Wang Y et al. Epithelial‑derived exosomes promote M2 macrophage polarization via Notch2/SOCS1 during mechanical ventilation. Int J Mol Med 50:N/A (2022). PubMed: 35616134
- Zhu P et al. Curcumin attenuates hypoxia/reoxygenation-induced cardiomyocyte injury by downregulating Notch signaling. Mol Med Rep 20:1541-1550 (2019). PubMed: 31257466
- Zhao D et al. Notch Signaling Pathway Regulates Angiogenesis via Endothelial Cell in 3D Co-Culture Model. J Cell Physiol 232:1548-1558 (2017). PubMed: 27861873
