Recombinant mouse IL-1 beta protein (Active)

• sELISA

Lab

Sandwich ELISA - Recombinant mouse IL-1 beta protein (Active) (AB259421)

Sandwich ELISA - Recombinant mouse IL-1 beta protein standard curve

Background subtracted standard curve using Mouse IL-1beta Antibody Pair - BSA and Azide free (ab241673) and Recombinant mouse IL-1 beta protein (Active) (ab259421) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.

• FuncS

Lab

Functional Studies - Recombinant mouse IL-1 beta protein (Active) (AB259421)

Fully biologically active compared to a standard. Determined by dose-dependant stimulation of human TF-1 cells. ED₅₀ is ≤ 0.5294 ng /ml, corresponding to a specific activity of 1.89 x 10⁶ units/mg.

• Mass Spec

Lab

Mass Spectrometry - Recombinant mouse IL-1 beta protein (Active) (AB259421)

M + 0.61 Da (Calc. mass 17451.39).

The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 μm, Thermo Scientific). 5 μL of purified protein was injected and the gradient run from 85 % water : FA (99.9 : 0.1 v/v) and 15 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) to 55 % water : FA (99.9 : 0.1 v/v) and 45 % acetonitrile : FA (90 : 9.9 : 0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).

• HPLC

Lab

HPLC - Recombinant mouse IL-1 beta protein (Active) (AB259421)

Purity 100%

The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 μm). 5 μL of purified protein was injected and the gradient run from 80 % water : TFA (99.9 : 0.1 v/v) and 20 % acetonitrile : water : TFA (90 : 9.9 : 0.1 v/v/v) to 20 % water : TFA (99.9 : 0.1 v/v) and 80 % acetonitrile : water : TFA (90 : 9.9 : 0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.

• SDS-PAGE

Lab

SDS-PAGE - Recombinant mouse IL-1 beta protein (Active) (AB259421)

SDS-PAGE analysis of ab259421.