Recombinant mouse IGF1 protein (Active) (ab9861)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品の詳細
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製品名
Recombinant mouse IGF1 protein (Active)
IGF1 タンパク質・ペプチド 製品一覧 -
生理活性
The ED50 , as determined a cell proliferation assay using FDC-P1 cells is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 105units/mg.
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精製度
>= 98 % SDS-PAGE.
>=98% HPLC analyses. Sterile filtered. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
配列
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA -
予測される分子量
8 kDa -
領域
49 to 118 -
配列の追加情報
Full length mature protein, without the signal peptide or the propeptides.
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特性
Our Abpromise guarantee covers the use of ab9861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
The ED50 was determined by a cell proliferation assay using FDC-P1 cells is < 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
関連情報
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別名
- IBP1
- IGF I
- IGF IA
see all -
機能
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. -
関連疾患
Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. -
配列類似性
Belongs to the insulin family. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab9861 は 6 報の論文で使用されています。
- Tan Y et al. Low-dose exercise protects the heart against established myocardial infarction via IGF-1-upregulated CTRP9 in male mice. MedComm (2020) 4:e411 (2023). PubMed: 38020715
- Cui X et al. D-4F Decreases White Matter Damage After Stroke in Mice. Stroke 47:214-20 (2016). PubMed: 26604250
- Cui X et al. Deficiency of brain ATP-binding cassette transporter A-1 exacerbates blood-brain barrier and white matter damage after stroke. Stroke 46:827-34 (2015). PubMed: 25593138
- Sabo JK et al. Investigation of sequential growth factor delivery during cuprizone challenge in mice aimed to enhance oligodendrogliogenesis and myelin repair. PLoS One 8:e63415 (2013). Functional Studies . PubMed: 23650566
- Xian L et al. Matrix IGF-1 maintains bone mass by activation of mTOR in mesenchymal stem cells. Nat Med 18:1095-101 (2012). PubMed: 22729283
- Li M et al. Down-regulation of manganese-superoxide dismutase through phosphorylation of FOXO3a by Akt in explanted vascular smooth muscle cells from old rats. J Biol Chem 281:40429-39 (2006). PubMed: 17079231