Recombinant mouse Hsp70 protein (ab113187)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant mouse Hsp70 protein
Hsp70 タンパク質・ペプチド 製品一覧 -
生理活性
ATPase activity assay (positive) but there is not quantification of activity
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精製度
> 85 % SDS-PAGE.
ab113187 is purified by multi-step chromatography. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
予測される分子量
70 kDa -
領域
1 to 641
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab113187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
Functional Studies
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 99% PBS, 0.15% Glutathione
This product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- DnaK type molecular chaperone HSP70 1
- Epididymis secretory protein Li 103
- FLJ54303
see all -
関連性
Function: In cooperation with other chaperones, the Hsp70 family stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell. Tissue specificity: HSPA1B is testis-specific. -
細胞内局在
Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
画像
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SDS-PAGE - Recombinant mouse Hsp70 protein (ab113187)SDS-PAGE showing ab113187 (1µg).
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Western blot - Recombinant mouse Hsp70 protein (ab113187)Lane 1: MWM
Lane 2: 100 ng ab113187
Lane 3: 50 ng E. coli DnaK Protein
Left: probed with Hsp70 mAb at 1.0 µg/ml.
Right: probed with DnaK mAb at 1.0 µg/ml.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab113187 は 2 報の論文で使用されています。
- Fan Y et al. Targeting multiple cell death pathways extends the shelf life and preserves the function of human and mouse neutrophils for transfusion. Sci Transl Med 13:N/A (2021). PubMed: 34321317
- Liu P et al. Targeting Extracellular Heat Shock Protein 70 Ameliorates Doxorubicin-Induced Heart Failure Through Resolution of Toll-Like Receptor 2-Mediated Myocardial Inflammation. J Am Heart Assoc 8:e012338 (2019). PubMed: 31576776
