Recombinant mouse GDNF protein (Animal Free) (ab187224)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant mouse GDNF protein (Animal Free)
GDNF タンパク質・ペプチド 製品一覧 -
生理活性
The activity is determined by the dose-dependent proliferation of C6 cells and is typically less than 1 μg/mL.
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精製度
>= 98 % SDS-PAGE.
Determined by reducing and Non-reducing SDS-PAGE -
エンドトキシン・レベル
<=1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
由来
Recombinant -
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生物種
Mouse -
配列
MSPDKQAALPRRENRNRQAAAASPENSRGKGRRGQRGKNRGCVLTAIHLN VTDLGLGYETKEELIFRYCSGSCESAETMYDKILKNLSRSRRLTSDKVGQ ACCRPVAFDDDLSFLDDNLVYHILRKHSAKRCGCI -
予測される分子量
30 kDa -
領域
78 to 211
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特性
Our Abpromise guarantee covers the use of ab187224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. DO NOT VORTEX. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
関連情報
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別名
- Astrocyte derived trophic factor
- Astrocyte derived trophic factor 1
- Astrocyte-derived trophic factor
see all -
機能
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. -
組織特異性
In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. -
関連疾患
Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. -
配列類似性
Belongs to the TGF-beta family. GDNF subfamily. -
細胞内局在
Secreted. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab187224 は論文での使用が確認できていません。
