Recombinant Mouse Ephrin B2 protein (Fc Chimera His Tag) (ab185427)
Key features and details
- Expression system: Mammalian
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE, HPLC
製品の詳細
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製品名
Recombinant Mouse Ephrin B2 protein (Fc Chimera His Tag)
Ephrin B2 タンパク質・ペプチド 製品一覧 -
精製度
> 95 % SDS-PAGE.
Purity of ab185427 was determined by SEC-HPLC and reducing SDS-PAGE. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
Mammalian -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
配列
RSIVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEY YKVYMVDKDQADRCTIKKENTPLLNCARPDQDVKFTIKFQEFSPNLWGLE FQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSAR NHGPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNLLGSEV DDIEGRMDEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTP EVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLT VLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREE MTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLY SKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH -
予測される分子量
50 kDa including tags -
領域
29 to 227 -
タグ
His tag C-Terminus , Fc tag C-Terminus -
配列の追加情報
Mouse protein fragment is fused with a FC-6His tag at the C terminus.
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab185427 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
HPLC
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -80°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.
pH: 7.40
Constituent: 100% PBS
Lyophilized from a 0.2 µM filtered solution. -
再構成Dissolve the lyophilized protein in 3X PBS. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
関連情報
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別名
- EFN B2
- EFNB 2
- Efnb2
see all -
機能
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
(Microbial infection) Acts as a receptor for Hendra virus and Nipah virus. -
組織特異性
Lung and kidney. -
配列類似性
Belongs to the ephrin family.
Contains 1 ephrin RBD (ephrin receptor-binding) domain. -
翻訳後修飾
Inducible phosphorylation of tyrosine residues in the cytoplasmic domain. -
細胞内局在
Membrane. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab185427 は論文での使用が確認できていません。
