Recombinant Mouse DLK-1 protein (Fc fragment) (ab108574)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 0.100 Eu/µg
- Tags: Fc tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant Mouse DLK-1 protein (Fc fragment)
DLK-1 タンパク質・ペプチド 製品一覧 -
生理活性
Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells. -
精製度
> 90 % SDS-PAGE.
ab108574 is 0.2µm filtered -
エンドトキシン・レベル
< 0.100 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
予測される分子量
75 kDa -
領域
1 to 305 -
タグ
Fc tag C-Terminus -
配列の追加情報
Fused with the Fc region of Human IgG1 at the C-terminus.
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特性
Our Abpromise guarantee covers the use of ab108574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
Working aliquots are stable for up to 3 months when stored at -20°C. Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: PBS
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再構成Reconstitute with 100µl sterile water.
関連情報
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別名
- Adipocyte differentiation inhibitor protein
- Brevideltin
- Delta like 1 homolog
see all -
機能
May have a role in neuroendocrine differentiation. -
組織特異性
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. -
配列類似性
Contains 6 EGF-like domains. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Membrane. - Information by UniProt
画像
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Functional Studies - Recombinant Mouse DLK-1 protein (Fc fragment) (ab108574)
Induction of SOX-9 with the treatment of recombinant mouse DLK-1-Fc (ab108574).
A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5µg/ml of ab108574 as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-rabbit Sox-9 and anti-rabbit GAPDH.1=0 min; 2=10 min; 3=30 min; 4=1 h; 5=2 h; 6=3 h; 7=4 h; 8=24 h.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab108574 は論文での使用が確認できていません。
