AB108574

Recombinant Mouse DLK-1 protein (Fc fragment)

Name: Recombinant Mouse DLK-1 protein (Fc fragment) (ab108574) | Abcam
Brand: Abcam Limited
SKU: AB108574
Price: 88000 JPY
Availability: InStock

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Recombinant Mouse DLK-1 protein (Fc fragment) is a Mouse Fragment protein, in the 1 to 305 aa range, expressed in HEK 293 cells, with >90%, < 0.1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

別名を表示する

Dlk, Pref1, Scp-1, Dlk1, Protein delta homolog 1, DLK-1, Adipocyte differentiation inhibitor protein, Preadipocyte factor 1, Pref-1

1 Images

Functional Studies - Recombinant Mouse DLK-1 protein (Fc fragment) (AB108574)

FuncS

Supplier Data

Functional Studies - Recombinant Mouse DLK-1 protein (Fc fragment) (AB108574)

Induction of SOX-9 with the treatment of recombinant mouse DLK-1-Fc (ab108574).
A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5μg/ml of ab108574 as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-rabbit Sox-9 and anti-rabbit GAPDH.

1=0 min; 2=10 min; 3=30 min; 4=1 h; 5=2 h; 6=3 h; 7=4 h; 8=24 h.

Key facts

精製度

>90% SDS-PAGE

ab108574 is 0.2µm filtered

エンドトキシンレベル

< 0.1 EU/µg

発現系

HEK 293 cells

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

アクセッション番号

Q09163

アニマルフリー

キャリアフリー

種

Mouse

再構成

Reconstitute in 100 µL of water

バッファー組成

Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"5 µg/mL", "notes":"<p></p>" } } }

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製品の詳細

Working aliquots are stable for up to 3 months when stored at -20°C. Inhibits adipogenesis of 3T3L-1 cells. Induces Sox-9 in 3T3L-1 cells.

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配列情報

[{"linker":null,"sequence":"","proteinLength":"Fragment","predictedMolecularWeight":"75 kDa","actualMolecularWeight":null,"aminoAcidEnd":305,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q09163","tags":[{"tag":"Human IgG1 Fc","terminus":"C-Terminus"}]}]

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出荷温度及び保存条件

製品の状態

Lyophilized

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Delta-like 1 homolog (DLK-1) also known as Pref-1 (preadipocyte factor 1) is a protein involved in a wide range of cellular processes. The mass of DLK-1 is approximately 65 kDa. This protein is expressed in various tissues including the liver brain and endocrine glands. DLK-1 has an important role in the regulation of cell differentiation and growth acting as a signal molecule that influences tissue development and regeneration.

Biological function summary

DLK-1 serves a function in the inhibition of adipocyte differentiation and the maintenance of preadipocyte phenotype. It does not form part of a complex but interacts with membrane-bound receptors to mediate signaling pathways that impact tissue development. Researchers study its role in embryonic development and its downregulation is a significant factor during the differentiation of mesenchymal cells. Due to these interactions DLK-1 has a pivotal involvement in processes governing tissue regeneration and growth control.

Pathways

Better understanding of DLK-1 involves examining its interaction in the Notch signaling pathway. This important pathway related to cell fate decisions features pathways with other proteins like Notch-1 and Notch-3. The relationship helps modulate the fate and proliferation of cells. DLK-1 also has associations with pathways involving phosphoinositide 3-kinase (PI3K) which influences cell growth and survival through related proteins such as AKT.

DLK-1 has shown associations with cancer and metabolic disorders. Overexpression of DLK-1 is linked to obesity due to its impact on preadipocyte differentiation. Additionally higher levels of DLK-1 can correlate with certain cancer progression where it acts alongside proteins like Notch-1 in promoting tumor cell growth. Understanding DLK-1's role in these contexts highlights the importance of DLK-1 as both a diagnostic marker and potential therapeutic target.

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一般的な情報

機能

May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.

翻訳後修飾

N- and O-glycosylated.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

May have a role in neuroendocrine differentiation. Inhibits adipocyte differentiation.

See full target information Dlk1

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Recombinant Mouse DLK-1 protein (Fc fragment)