Recombinant mouse DKK1 protein (Active) (ab281791)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies
製品の詳細
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製品名
Recombinant mouse DKK1 protein (Active)
DKK1 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active measured by its ability to inhibit Wnt3-a induced alkaline phosphatase production by MC3T3-E1 mouse pre-osteoblast cells.
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精製度
>= 95 % SDS-PAGE.
>=95% Purity by HPLC -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Mouse -
配列
TLNSVLINSNAIKNLPPPLGGAGGQPGSAVSVAPGVLYEGGNKYQTLDNY QPYPCAEDEECGSDEYCSSPSRGAAGVGGVQICLACRKRRKRCMRHAMCC PGNYCKNGICMPSDHSHFPRGEIEESIIENLGNDHNAAAGDGYPRRTTLT SKIYHTKGQEGSVCLRSSDCAAGLCCARHFWSKICKPVLKEGQVCTKHKR KGSHGLEIFQRCYCGEGLACRIQKDHHQASNSSRLHTCQRH -
予測される分子量
29 kDa -
領域
32 to 272 -
配列の追加情報
N-terminal glycine.
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特性
Our Abpromise guarantee covers the use of ab281791 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- Dickkopf 1
- Dickkopf 1 homolog
- Dickkopf 1 like
see all -
機能
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
組織特異性
Placenta. -
配列類似性
Belongs to the dickkopf family. -
ドメイン
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
細胞内局在
Secreted. - Information by UniProt
画像
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Fully biologically active measured by its ability to inhibit Wnt3-a induced alkaline phosphatase production in C2C12 cells.
ED50 for this effect is ≤5.9 ng/ml corresponding to specific activity of 1.69 x 105 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot # GR3397988-1.
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SDS-Page anaylsis of ab281791.
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HPLC analysis of ab281791.
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Mass determination by ESI-TOF.
Predicted MW is 26212.64 Da. (+/- 10 Da by ESI-TOF). Observes MW is 26213.93 Da. Additional masses are due to residual O-glycans.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab281791 は論文での使用が確認できていません。