JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB281791

Recombinant mouse DKK1 protein (Active)

Recombinant mouse DKK1 protein (Active)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant mouse DKK1 protein (Active) is a Mouse Full Length protein, in the 32 to 272 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for FuncS.

別名を表示する

Dickkopf-related protein 1, Dickkopf-1, Dkk-1, mDkk-1, Dkk1

4 Images
Functional Studies - Recombinant mouse DKK1 protein (Active) (AB281791)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse DKK1 protein (Active) (AB281791)

Fully biologically active measured by its ability to inhibit Wnt3-a induced alkaline phosphatase production in C2C12 cells.

ED50 for this effect is ≤5.9 ng/ml corresponding to specific activity of 1.69 x 105 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot # GR3397988-1.

Mass Spectrometry - Recombinant mouse DKK1 protein (Active) (AB281791)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant mouse DKK1 protein (Active) (AB281791)

Mass determination by ESI-TOF.

Predicted MW is 26212.64 Da. (+/- 10 Da by ESI-TOF). Observes MW is 26213.93 Da. Additional masses are due to residual O-glycans.

SDS-PAGE - Recombinant mouse DKK1 protein (Active) (AB281791)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse DKK1 protein (Active) (AB281791)

SDS-Page anaylsis of ab281791.

HPLC - Recombinant mouse DKK1 protein (Active) (AB281791)
  • HPLC

Supplier Data

HPLC - Recombinant mouse DKK1 protein (Active) (AB281791)

HPLC analysis of ab281791.

Key facts

精製度

>95% SDS-PAGE

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

タグ

Tag free

アプリケーション

FuncS

applications

生物活性

Yes

生物学的活性

Fully biologically active measured by its ability to inhibit Wnt3-a induced alkaline phosphatase production by MC3T3-E1 mouse pre-osteoblast cells.

アクセッション番号

O54908

アニマルフリー

No

キャリアフリー

No

種

Mouse

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

配列情報

[{"sequence":"TLNSVLINSNAIKNLPPPLGGAGGQPGSAVSVAPGVLYEGGNKYQTLDNYQPYPCAEDEECGSDEYCSSPSRGAAGVGGVQICLACRKRRKRCMRHAMCCPGNYCKNGICMPSDHSHFPRGEIEESIIENLGNDHNAAAGDGYPRRTTLTSKIYHTKGQEGSVCLRSSDCAAGLCCARHFWSKICKPVLKEGQVCTKHKRKGSHGLEIFQRCYCGEGLACRIQKDHHQASNSSRLHTCQRH","proteinLength":"Full Length","predictedMolecularWeight":"29 kDa","actualMolecularWeight":null,"aminoAcidEnd":272,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"O54908","tags":[]}]
style
left-column

出荷温度及び保存条件

出荷温度
Ambient - Can Ship with Ice
短期保存温度
Ambient
長期保存温度
Ambient
True
style
left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.
Biological function summary

The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.

Pathways

DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.

Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.
style
left-column

製品の性状

製品の状態

Lyophilized

補足情報

>=95% Purity by HPLC

style
left-column

一般的な情報

機能

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed : 18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed : 26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed : 18505822).

配列の類似性

Belongs to the dickkopf family.

style
left-column

製品プロトコール

style
left-column

ターゲットの情報

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed : 18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed : 26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed : 18505822).
See full target information Dkk1
style
left-column
style
left-column
style
right-column

組合せ製品

Proteins & Peptides

AB259410

Recombinant human TNF alpha protein (Active)

Premium Bioactive

Flow Cytometry - Recombinant human TNF alpha protein (Active) (AB259410)

  • 0
  • 0
Proteins & Peptides

AB9796

Recombinant human NGF protein (Active)

Bioactive

  • 0
  • 0
Proteins & Peptides

AB280347

Recombinant human MMP2 protein (Active)

Premium Bioactive

Functional Studies - Recombinant human MMP2 protein (Active) (AB280347)

  • 0
  • 0
Proteins & Peptides

AB283424

Recombinant Human MMP1 protein

SDS-PAGE - Recombinant Human MMP1 protein (AB283424)

  • 0
  • 0

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com