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AB219240

Recombinant Mouse Cathepsin B protein (His tag C-Terminus)

Recombinant Mouse Cathepsin B protein (His tag C-Terminus)

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Recombinant Mouse Cathepsin B protein (His tag C-Terminus) is a Mouse Full Length protein, in the 18 to 339 aa range, expressed in Baculovirus infected insect cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE.

別名を表示する

Cathepsin B, Cathepsin B1, Ctsb

1 Images
SDS-PAGE - Recombinant Mouse Cathepsin B protein (His tag) (AB219240)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse Cathepsin B protein (His tag) (AB219240)

15% SDS-PAGE analysis of 3 μg ab219240.

Molecular Weight : 28-40kDa (SDS-PAGE under reducing conditions).

Key facts

精製度

>90% SDS-PAGE

エンドトキシンレベル

< 1 EU/µg

発現系

Baculovirus infected insect cells

タグ

His tag C-Terminus

アプリケーション

SDS-PAGE

applications

生物活性

No

アクセッション番号

P10605

アニマルフリー

No

キャリアフリー

No

Mouse

バッファー組成

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

配列情報

[{"sequence":"HDKPSFHPLSDDLINYINKQNTTWQAGRNFYNVDISYLKKLCGTVLGGPKLPGRVAFGEDIDLPETFDAREQWSNCPTIGQIRDQGSCGSCWAFGAVEAISDRTCIHTNGRVNVEVSAEDLLTCCGIQCGDGCNGGYPSGAWSFWTKKGLVSGGVYNSHVGCLPYTIPPCEHHVNGSRPPCTGEGDTPRCNKSCEAGYSPSYKEDKHFGYTSYSVSNSVKEIMAEIYKNGPVEGAFTVFSDFLTYKSGVYKHEAGDMMGGHAIRILGWGVENGVPYWLAANSWNLDWGDNGFFKILRGENHCGIESEIVAGIPRTDQYWGRFLEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"36.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":339,"aminoAcidStart":18,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"P10605","tags":[{"tag":"His","terminus":"C-Terminus"}]}]

出荷温度及び保存条件

出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cathepsin B also known as CTSB or cathepsin beta is a lysosomal cysteine protease belonging to the papain family. This enzyme possesses a molecular weight of approximately 25 to 30 kDa. You can find cathepsin B expressed in various tissues with high levels observed in the liver kidney and spleen. It plays a mechanical role in protein degradation within lysosomes operating optimally in acidic environments. The enzyme has active and inactive forms depending on structural modifications and environmental conditions.
Biological function summary

Cathepsin B participates as a proteolytic enzyme involved in protein turnover and processing. It does not function in isolation but often associates with various other proteases forming complexes essential for efficient substrate breakdown. Cathepsin B assists in the activation of other enzymes by cleaving propeptides therefore facilitating the initiation of cascade reactions. This role is critical in maintaining cellular homeostasis and modulating physiological processes including cell migration and immune response modulation.

Pathways

Cathepsin B significantly contributes to the lysosomal protein degradation pathway and the autophagy-lysosome pathway. It interacts with proteins such as B protein which are important for cellular recycling and energy homeostasis. Its activity supports the MHC class II antigen processing pathway playing an essential part in immune system functionality. Cathepsin B also exhibits interactions with Ctsb protein inhibitors which regulate its proteolytic activity within these pathways.

Cathepsin B has strong associations with cancer progression and neurological disorders such as Alzheimer's disease. Its overexpression and dysregulation are linked to tumor metastasis where it degrades extracellular matrix components promoting invasion. Ctsb protein interactions with corey mazo proteins influence tumor microenvironment and immune evasion. In Alzheimer's disease cathepsin B can influence amyloid beta peptide processing connected to neurodegenerative pathology progression. These roles highlight the potential for cathepsin B as a therapeutic target in addressing these conditions.

製品の性状

製品の状態

Liquid

補足情報

Affinity purified

一般的な情報

機能

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (By similarity). Cleaves matrix extracellular phosphoglycoprotein MEPE (By similarity). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (PubMed : 12782676). Has also been implicated in tumor invasion and metastasis (By similarity).

配列の類似性

Belongs to the peptidase C1 family.

細胞内局在性

Lysosome

製品プロトコール

ターゲットの情報

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins (By similarity). Cleaves matrix extracellular phosphoglycoprotein MEPE (By similarity). Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (PubMed : 12782676). Has also been implicated in tumor invasion and metastasis (By similarity).
See full target information CATB_MOUSE

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