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Recombinant mouse C10 protein (ab9898)

Submit a review Q&A (1)
Western blot - Recombinant mouse C10 protein (ab9898)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 99% SDS-PAGE
    • Active: Yes
    • Suitable for: Functional Studies, SDS-PAGE, WB

    製品の詳細

    • 製品名

      Recombinant mouse C10 protein
      C10 タンパク質・ペプチド 製品一覧

    • 精製度

      > 99 % SDS-PAGE.
      Sterile filtered Greater than 99% pure by HPLC analyses. Endotoxin level is less than 0.1 ng per g (1EU/g).

    • 発現系

      Escherichia coli

    • アクセッション番号

      P27784

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Mouse

      • 配列

        GLIQEIEKEDRRYNPPIIHQGFQDTSSDCCFSYATQIPCKRFIYYFPTSG GCIKPGIIFISRRGTQVCADPSDRRVQRCLSTLKQGPRSGNKVIA

      • 予測される分子量

        11 kDa

      • 領域

        22 to 116

    特性

    Our Abpromise guarantee covers the use of ab9898 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      Functional Studies

      SDS-PAGE

      Western blot

    • 製品の状態

      Lyophilized

    • 備考

      The biological activity of this product was determined by its ability to chemoattract Balb/c mouse spleen MNCs using a concentration of 10-100ng/ml. 

    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • 再構成
      For lot specific reconstitution information please contact our Scientific Support Team.

    関連情報

    • 別名

      • C10
      • CCL 6
      • chemokine (C-C motif) ligand 6
      • Chemokine C C motif Ligand 6
      • MRP1
      • SCYA6
      • Small Inducible Cytokine A6
      see all

    • 関連性

      CCL6 (C10) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It is a potent chemoattractant of macrophages, but it can also attract B cells, CD4+ lymphocytes and eosinophils. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be induced under conditions suitable for myeloid cell differentiation. CCL6 (C10) was originally identified as a transcript that is induced in bone marrow cells upon stimulation of GMCSF. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1.

    • 細胞内局在

      Secreted

    画像

    • Western blot - Recombinant mouse C10 protein (ab9898)
      Western blot - Recombinant mouse C10 protein (ab9898)
      All lanes : Anti-C10 antibody (ab9897) at 0.2 µg/ml

      Lane 1 : Recombinant mouse C10 protein (ab9898) at 0.1 µg
      Lane 2 : Recombinant mouse C10 protein (ab9898) at 0.01 µg

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 1 minute

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab9898 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9898 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Question

    My question is whether the "highly pure recombinant mouse CCL6" you used to immunize the animals is:
    (1) complete full length protein containing N-terminal signal peptide (a.a 1-21)?,
    (2) secreted protein lacking signal peptide but maintain whole other parts (a.a 22-116)? or
    (3) mature active protein lacking the N-terminal domain (22-43), which contains only a.a. 44-116?
    I can agree with your statement that epitope mapping is usually not performed for validating poly-clonal antibodies.
    So the details of the immunogen is quite important to estimate the reactivity of the antibody.
    If (3) above is the case, we don't have to worry about the reactivity because this antibody must react with all forms of CCL6.
    But if (1) or (2) is the case, it might happen that this antibody preferentially react with inactive form of CCL6 with N-terminal a.a. 1-43 or 22-43.
    So if possible, please give me the details of recombinant CCL6 used as an immunogen.
    Regards,

    Read More
    Answer

    Thank you for your enquiry.

    The recombinant MurineCCL6 (C10) is also in our catalogue ab9898. This is the mature protein, which contains 95 amino acids (amino acids 22-116).

    The amino acid sequence is as follows:

    GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA

    The 21 amino acid N-terminal signal peptide is not present on the protein.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

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