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AB316677

Recombinant Mouse Annexin A1 Protein Standard (His tag)

Recombinant Mouse Annexin A1 Protein Standard (His tag)

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Recombinant Mouse Annexin A1 Protein Standard (His tag) is a Mouse Fragment protein, expressed in HEK 293 cells, with >80%, suitable for sELISA, SDS-PAGE.

別名を表示する

Anx1, Lpc-1, Lpc1, Anxa1, Annexin A1, Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35

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Sandwich ELISA - Recombinant Mouse Annexin A1 Protein Standard (His tag) (AB316677)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Mouse Annexin A1 Protein Standard (His tag) (AB316677)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Mouse Annexin A1 Protein Standard (His tag) (AB316677)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse Annexin A1 Protein Standard (His tag) (AB316677)

SDS-PAGE analysis of ab316677 under reducing conditions for 2ug protein.

Key facts

精製度

>80% SDS-PAGE

発現系

HEK 293 cells

タグ

His tag C-Terminus

アプリケーション

sELISA, SDS-PAGE

applications

生物活性

No

アクセッション番号

P10107

アニマルフリー

Yes

キャリアフリー

No

種

Mouse

バッファー組成

pH: 7.3 - 7.5 Constituents: 2.922% Sodium chloride, 0.64107% disodium;hydrogen phosphate;dodecahydrate, 0.02858% Potassium phosphate monobasic

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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製品の詳細

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.

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配列情報

[{"sequence":null,"proteinLength":"Fragment","predictedMolecularWeight":"40.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P10107","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Annexin A1 also known as ANXA1 or annexin 1 is a multifunctional protein with a molecular mass of approximately 37 kDa. This protein belongs to the annexin family and is distributed widely in various tissue types including the central nervous system respiratory system and immune cells. Annexin A1 functions by binding to phospholipids in a calcium-dependent manner influencing membrane-related processes and signal transduction. Its expression can be examined using techniques like annexin A1 immunohistochemistry and annexin A1 ELISA to study protein localization and concentration.
Biological function summary

Annexin A1 is involved in modulating inflammatory responses and cell proliferation. It acts as a mediator in the resolution of inflammation by promoting apoptotic cell clearance and regulating leukocyte migration. Annexin A1 participates in complex interactions often associating with cell membrane phospholipids to exert its effects. It contributes significantly to cell adhesion migration and intracellular signaling events which are important for maintaining homeostasis and proper immune function.

Pathways

Annexin A1 plays a significant role in the glucocorticoid signaling pathway and the formation of the cytoskeleton. It interacts with proteins like lipocortin 1 and phospholipase A2 impacting processes such as arachidonic acid metabolism and cytoskeletal reorganization. These interactions underline annexin A1's involvement in regulating inflammation and maintaining cellular architecture linking it to cellular processes that govern stress responses and cell movement.

Annexin A1 exhibits relevance to inflammatory diseases and cancer. It has been implicated in conditions such as asthma where it modulates inflammatory cell recruitment and activation. In cancer especially in breast cancer annexin A1 influences tumor progression by interacting with proteins like COX-2 affecting tumor cell invasion and metastasis. The protein's role in these conditions highlights its potential as both a biomarker and therapeutic target helping in understanding disease mechanisms and developing treatment strategies.
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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed : 12475898). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (PubMed : 12475898). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed : 17948261). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed : 17948261). Has no effect on unstimulated T-cells. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (By similarity). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (By similarity). Plays a role in the formation of phagocytic cups and phagosomes (PubMed : 21245195). Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (PubMed : 21245195).. Annexin Ac2-26. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Promotes resolution of inflammation and wound healing. Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2.

配列の類似性

Belongs to the annexin family.

翻訳後修飾

Phosphorylated by protein kinase C, EGFR and TRPM7. Phosphorylated in response to EGF treatment.. Sumoylated.. Proteolytically cleaved by cathepsin CTSG to release the active N-terminal peptide Ac2-26.

細胞内局在性

Nucleus

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製品プロトコール

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ターゲットの情報

Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed : 12475898). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (PubMed : 12475898). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed : 17948261). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed : 17948261). Has no effect on unstimulated T-cells. Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (By similarity). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (By similarity). Plays a role in the formation of phagocytic cups and phagosomes (PubMed : 21245195). Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (PubMed : 21245195).. Annexin Ac2-26. Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades. Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors. Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration. Promotes resolution of inflammation and wound healing. Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2.
See full target information Anxa1
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