AB227417

Recombinant mouse AK2 protein (Active)

Name: Recombinant mouse AK2 protein (Active) (ab227417) | Abcam
Brand: Abcam Limited
SKU: AB227417
Price: 41000 JPY
Availability: InStock

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Recombinant mouse AK2 protein (Active) is a Mouse Full Length protein, in the 1 to 239 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS, Mass Spec.

別名を表示する

AK 2, ATP-AMP transphosphorylase 2, ATP:AMP phosphotransferase, Adenylate monophosphate kinase, Ak2

2 Images

SDS-PAGE - Recombinant mouse AK2 protein (Active) (AB227417)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse AK2 protein (Active) (AB227417)

15% SDS-PAGE analysis of 3µg ab227417 .

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse AK2 protein (Active) (AB227417)

15% SDS-PAGE analysis of 3µg ab227417

Key facts

精製度

>95% SDS-PAGE

発現系

Escherichia coli

アプリケーション

FuncS, Mass Spec, SDS-PAGE

applications

生物活性

Yes

生物学的活性

Specific activity is > 40 units/mg. One unit will convert 2.0 μmoles of ADP to ATP + AMP per minute at pH 7.5 at 37°C.

アクセッション番号

Q9WTP6

アニマルフリー

キャリアフリー

種

Mouse

バッファー組成

pH: 8.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSHMAPNVLASEPEIPKGIRAVLLGPPGAGKGTQAPKLAENFCVCHLATGDMLRAMVASGSELGKKLKATMDAGKLVSDEMVVELIEKNLETPSCKNGFLLDGFPRTVRQAEMLDDLMEKRKEKLDSVIEFSIQDSLLIRRITGRLIHPKSGRSYHEEFNPPKEPMKDDITGEPLIRRSDDNEKALKTRLEAYHTQTTPLVEYYRKRGIHCAIDASQTPDIVFASILAAFSKATCKDLVMFI","proteinLength":"Full Length","predictedMolecularWeight":"29.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":239,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9WTP6","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The adenylate kinase 2 also known as AK2 is an enzyme that catalyzes the conversion of adenine nucleotides within the cell. It facilitates the reversible conversion of ADP into ATP and AMP playing a critical role in cellular energy homeostasis. AK2 weighing approximately 26 kDa is primarily found in the mitochondrial intermembrane space of various tissues including liver muscle and brain. Its expression allows for efficient energy transfer essential for normal mitochondrial function and cellular metabolism.

Biological function summary

AK2 functions in energy transfer and regulation in the cell serving as an important component in maintaining cellular ATP levels. AK2 is an integral part of the mitochondrial adenylate kinase system which manages energy flux in cells. By balancing the concentrations of ATP ADP and AMP AK2 directly influences cellular energy state and metabolic activity. The enzyme also supports nucleotide homeostasis therefore facilitating processes such as signal transduction and nucleic acid synthesis.

Pathways

AK2 is involved in the adenylate kinase pathway important for energy metabolism. It coordinates with other enzymes like AK1 and AK3 ensuring efficient energy distribution in the cell. AK2 also interfaces with the apoptosis signaling pathways where it plays a part in regulating programmed cell death. The interaction of AK2 within these pathways emphasizes its essential role in maintaining cellular energy balance and undergoing apoptosis when necessary.

AK2 mutations link to rare conditions like reticular dysgenesis a severe form of combined immunodeficiency. The disorder arises when AK2 fails to initiate proper energy transfer necessary for white blood cell development. AK2 is also related to mitochondrial disorders where energy production gets critically impaired manifesting in muscle weakness and neurodegenerative symptoms. The enzyme's connection to proteins involved in energy pathways suggests its potential contribution to the progression of such diseases.

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製品の性状

製品の状態

Liquid

補足情報

ab227417 was purified using conventional chromatography techniques.

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一般的な情報

機能

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.

配列の類似性

Belongs to the adenylate kinase family. AK2 subfamily.

細胞内局在性

Mitochondrion intermembrane space

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製品プロトコール

Visit the General protocols
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ターゲットの情報

See full target information Ak2

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Abcam product promise

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Recombinant mouse AK2 protein (Active)