JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB199750

Recombinant mCherry protein (His tag)

Recombinant mCherry protein (His tag)

4

(1 Review)

|

(2 Publications)

Recombinant mCherry protein (His tag) is a Full Length protein, in the 1 to 236 aa range with >97% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE and HPLC. The predicted molecular weight of ab199750 protein is 28.8 kDa.

- Save time and ensure accurate results - use our mCherry protein (His tag) protein as a control

Key facts

精製度

>97% SDS-PAGE

Greater than or equal to 97% by HPLC.

エンドトキシンレベル

< 1 EU/µg

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

SDS-PAGE, HPLC

applications

生物活性

No

アニマルフリー

No

キャリアフリー

No

再構成

Reconstitute at 1 mg/mL in water

バッファー組成

pH: 7.4 Constituents: 10.269% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

製品の詳細

Ensure the validity of your result using our recombinant mCherry protein (His tag) protein ab199750 as a control in SDS-PAGE.

Function

mCherry is the second generation monomeric red fluorescent protein that has improved brightness and photostability. The protein is suitable as a positive control for mCherry protein expression studies or as a labeling reagent. The recombinant mCherry protein ab199750 is ideal for fusion tag applications and is perfect for triple labeling with EGFP, CFP, YFP, or any other dyes.

The protein is engineered with 6xHis-tag on the N-terminus, which can be used for detection with anti-His-Tag antibody or protein purification/removal by using Ni++ beads.


Endotoxin level: <0.1 ng/µg


Check out our protein gel staining guide for SDS-PAGE here


This product is manufactured by BioVision, an Abcam company and was previously called 4993 mCherry Fluorescent Protein. 4993-100 is the same size as the 100 µg size of ab199750.

配列情報

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK","proteinLength":"Full Length","predictedMolecularWeight":"28.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":236,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":null,"tags":[{"tag":"His","terminus":"N-Terminus"}]}]

出荷温度及び保存条件

製品の状態
Lyophilized
出荷温度
Blue Ice
短期保存温度
-80°C
長期保存温度
-80°C
False

一般的な情報

製品プロトコール

ターゲットの情報

See full target information mCherry

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Vaccines 12: PubMed39591176

2024

Vaccination Against Androgen Receptor Splice Variants to Immunologically Target Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Robert D Marek,Selena Halabi,Mu-En Wang,Jason McBane,Junping Wei,Tao Wang,Xiao Yang,Congxiao Liu,Gangjun Lei,Herbert Kim Lyerly,Ming Chen,Timothy N Trotter,Zachary C Hartman

Biochemical and biophysical research communication 516:914-920 PubMed31272717

2019

Inhibition of allogeneic islet graft rejection by VISTA-conjugated liposome.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Guo,Shu Han,Yanfang Liu,Wenyuan Guo,Yuanyu Zhao,Fang Liu,Xiaomin Shi,Guoshan Ding,Quanxing Wang
View all publications

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com