Recombinant listeria monocytogenes Listeriolysin (LLO) protein (ab83345)

Thank you for confirming the suggestions helped your customer to get the expected results. That is good news.

Have a nice day.

Thank you for contacting us. I am sorry to hear that this protein is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed this case, our collaborators from the lab where the protein is producedoffered some suggestions to help optimize the results from ab83345:

"The procedures require that the hemolytic activity of each new batch of listeriolysin O PEST-minus is determined by the assay described in Giammarini C. et al. "Purification and characterization of a recombinant listeriolysin O expressed in Escherichia coli and possible diagnostic applications", .J Biotechnol. 2004 Apr 8;109(1-2):13-20.
In particular, listeriolysin O PEST minus lotGR61074 has provided the result of 7x10(4) HU/mg, that corresponds to 1HU = 14.2ng.
Unfortunately we have no information on the performance of our product in the specific application described by the customer".

Should the suggestions not improve the results, please do let me know.

In the event that a product is not functioning in the species and applications cited on the product datasheet (and the problem has been reported within 6 months of purchase), we would be pleased to provide a free of charge replacement, credit note, or refund.

I hope this information is helpful, and I thank you for your cooperation.

Thank you for contacting us.


I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

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I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.