AB190125

Recombinant Influenza A Virus Hemagglutinin H5 protein

Name: Recombinant Influenza A Virus Hemagglutinin H5 protein (ab190125) | Abcam
Brand: Abcam Limited
SKU: AB190125
Price: 245000 JPY
Availability: InStock

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(4 Publications)

Recombinant Influenza A Virus Hemagglutinin H5 protein is a Full Length protein, in the 1 to 567 aa range, expressed in Baculovirus infected Sf9 cells, with >80%, suitable for Agg, WB, SDS-PAGE.

別名を表示する

Hemagglutinin 5, H5HA

3 Images

Supplier Data

Western blot - Recombinant Influenza A Virus Hemagglutinin H5 protein (AB190125)

All lanes:

anti-H5 ferret antiserum at 1/20 dilution

All lanes:

Western blot - Recombinant Influenza A Virus Hemagglutinin H5 protein (ab190125) at 0.2 µg

Secondary

All lanes:

an anti-ferret AP conjugate followed by substrate.

false

SDS-PAGE - Recombinant Influenza A Virus Hemagglutinin H5 protein (AB190125)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Influenza A Virus Hemagglutinin H5 protein (AB190125)

SDS-PAGE analysis of 1.5 μg of ab190125 protein under reducing conditions.

Supplier Data

Agglutination - Recombinant Influenza A Virus Hemagglutinin H5 protein (AB190125)

Hemagglutination with turkey red blood cells : ab190125 titer 1 : 8192

Key facts

精製度

>80% SDS-PAGE

Affinity purified by FPLC. Residual baculovirus GP64 co-purifies with the affinity purified ab190125 and was determined to be less the 25% of the total protein.

発現系

Baculovirus infected Sf9 cells

アプリケーション

Agg, SDS-PAGE, WB

applications

生物活性

アニマルフリー

キャリアフリー

Yes

バッファー組成

Constituents: 99% Tris buffered saline

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Agg": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Quality control testing demonstrates detection of GP null under reduced conditions when using anti-H5 influenza antiserum." }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms." } } }

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配列情報

[{"linker":null,"sequence":"MEKIVLLFAIVSLVKSDQICIGYHANNSTEQVDTIMEKNVTVTHAQDILEKKHNGKLCDLDGVKPLILRDCSVAGWLLGNPMCDEFINVPEWSYIVEKANPVNDLCYPGDFNDYEELKHLLSRINHFEKIQIIPKSSWSSHEASLGVSSACPYQGKSSFFRNVVWLIKKNSTYPTIKRSYNNTNQEDLLVLWGIHHPNDAAEQTKLYQNPTTYISVGTSTLNQRLVPRIATRSKVNGQSGRMEFFWTILKPNDAINFESNGNFIAPEYAYKIVKKGDSTIMKSELEYGNCNTKCQTPMGAINSSMPFHNIHPLTIGECPKYVKSNRLVLATGLRNSPQRERRRKKRGLFGAIAGFIEGGWQGMVDGWYGYHHSNEQGSGYAADKESTQKAIDGVTNKVNSIIDKMNTQFEAVGREFNNLERRIENLNKKMEDGFLDVWTYNAELLVLMENERTLDFHDSNVKNLYDKVRLQLRDNAKELGNGCFEFYHKCDNECMESVRNGTYDYPQYSEEARLKREEISGVKLESIGIYQILSIYSTVASSLALAIMVAGLSLWMCSNGSLQCRICI","proteinLength":"Full Length","predictedMolecularWeight":"64 kDa","actualMolecularWeight":null,"aminoAcidEnd":567,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":null,"tags":[]}]

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

出荷温度

Dry Ice

短期保存期間

1-2 weeks

短期保存温度

-20°C

長期保存温度

-80°C

保管に関する情報

Avoid freeze / thaw cycle

False

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一般的な情報

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information Influenza A Virus Hemagglutinin H5

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Microorganisms 10: PubMed35208815

2022

Transformation of by for the Expression of the Hemagglutinin of Avian Influenza Virus H5.

Applications

Unspecified application

Species

Unspecified reactive species

Inkar Castellanos-Huerta,Gabriela Gómez-Verduzco,Guillermo Tellez-Isaias,Guadalupe Ayora-Talavera,Bernardo Bañuelos-Hernández,Víctor Manuel Petrone-García,Gilberto Velázquez-Juárez,Isidro Fernández-Siurob

International journal of molecular sciences 22: PubMed33673708

2021

The Functional Role of Loops and Flanking Sequences of G-Quadruplex Aptamer to the Hemagglutinin of Influenza a Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasia A Bizyaeva,Dmitry A Bunin,Valeria L Moiseenko,Alexandra S Gambaryan,Sonja Balk,Vadim N Tashlitsky,Alexander M Arutyunyan,Alexey M Kopylov,Elena G Zavyalova

Methods in molecular biology (Clifton, N.J.) 2132:567-583 PubMed32306357

2020

Preparation and Detection of Glycan-Binding Activity of Influenza Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Shin-Ichi Nakakita,Nongluk Sriwilaijaroen,Yasuo Suzuki,Jun Hirabayashi

Biomolecules 10: PubMed31936820

2020

Structural and Functional Aspects of G-Quadruplex Aptamers Which Bind a Broad Range of Influenza A Viruses.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasia A Novoseltseva,Nikita M Ivanov,Roman A Novikov,Yaroslav V Tkachev,Dmitry A Bunin,Alexandra S Gambaryan,Vadim N Tashlitsky,Alexander M Arutyunyan,Alexey M Kopylov,Elena G Zavyalova

View all publications

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Recombinant Influenza A Virus Hemagglutinin H5 protein