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AB307788

Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag)

Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag)

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Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) is a Influenza A virus (A/Puerto Rico/8/1934(H1N1)) Fragment protein, in the 18 to 528 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for HPLC, SDS-PAGE, Mass Spec.

別名を表示する

Hemagglutinin, HA

3 Images
Mass Spectrometry - Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) (AB307788)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) (AB307788)

Mass determination by ESI-TOF. Predicted MW is 59263.11 Da (+/- 10 Da by ESI-TOF). Observed MW is 59269.29 Da.

HPLC - Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) (AB307788)
  • HPLC

Supplier Data

HPLC - Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) (AB307788)

HPLC analysis of ab307788

SDS-PAGE - Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) (AB307788)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Influenza A H1N1 Hemagglutinin Protein (His-tag) (AB307788)

SDS-PAGE analysis of ab307788

Key facts

精製度

>95% HPLC

SDS-PAGE >= 95%

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

タグ

His tag C-Terminus

アプリケーション

SDS-PAGE, HPLC, Mass Spec

applications

生物活性

No

アクセッション番号

P03452

アニマルフリー

Yes

キャリアフリー

No

種

Influenza A virus (A/Puerto Rico/8/1934(H1N1))

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB325995

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 11 bands) (AB325995)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB273530

Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag)

ELISA - Recombinant Human coronavirus SARS-CoV-2 nucleocapsid protein (His tag) (AB273530)

  • 0
  • 0
Primary Antibodies

AB236632

Anti-HA tag antibody [EPR22819-101]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HA tag antibody [EPR22819-101] (AB236632)

  • 3
  • 1
Proteins & Peptides

AB190125

Recombinant Influenza A Virus Hemagglutinin H5 protein

Western blot - Recombinant Influenza A Virus Hemagglutinin H5 protein (AB190125)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"DTICIGYHANNSTDTVDTVLEKNVTVTHSVNLLEDSHNGKLCRLKGIAPLQLGKCNIAGWLLGNPECDPLLPVRSWSYIVETPNSENGICYPGDFIDYEELREQLSSVSSFERFEIFPKESSWPNHNTNGVTAACSHEGKSSFYRNLLWLTEKEGSYPKLKNSYVNKKGKEVLVLWGIHHPPNSKEQQNLYQNENAYVSVVTSNYNRRFTPEIAERPKVRDQAGRMNYYWTLLKPGDTIIFEANGNLIAPMYAFALSRGFGSGIITSNASMHECNTKCQTPLGAINSSLPYQNIHPVTIGECPKYVRSAKLRMVTGLRNIPSIQSRGLFGAIAGFIEGGWTGMIDGWYGYHHQNEQGSGYAADQKSTQNAINGITNKVNTVIEKMNIQFTAVGKEFNKLEKRMENLNKKVDDGFLDIWTYNAELLVLLENERTLDFHDSNVKNLYEKVKSQLKNNAKEIGNGCFEFYHKCDNECMESVRNGTYDYPKYSEESKLNREKVDGVKLESMGIYQ","proteinLength":"Fragment","predictedMolecularWeight":"59.26 kDa","actualMolecularWeight":"59.27 kDa","aminoAcidEnd":528,"aminoAcidStart":18,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P03452","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Lyophilized
出荷温度
Blue Ice
短期保存温度
Ambient
長期保存温度
Ambient
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Influenza A Virus Hemagglutinin also known as hemagglutinin or influenza HA is a surface glycoprotein found on the influenza A virus. Hemagglutinin has a mass of approximately 75 kDa and serves as an important player in the virus's ability to infect host cells. It is expressed on the exterior membrane of the influenza virus and allows the virus to attach to and enter host cells initiating the infection process. This protein comes in various subtypes adding complexity to the virus's infection strategies and complicating vaccine development.
Biological function summary

Hemagglutinin facilitates the binding of the virus to sialic acid receptors on the surface of host epithelial cells. This binding triggers endocytosis allowing the virus entry into the host cell. Hemagglutinin is part of a complex that works closely with neuraminidase another glycoprotein on the virus surface. The hemagglutinin-neuraminidase complex is essential for the viral life cycle balancing attachment to host cells with the release of new viral particles.

Pathways

Hemagglutinin plays a significant role in the viral infection pathway of the influenza virus. It is critical in the initial steps of the pathway that involve viral entry and fusion with the host cell membrane. Hemagglutinin's interaction with the sialic acid receptors is closely linked with the activity of neuraminidase which aids in the release stage of the infection. This interplay between hemagglutinin and neuraminidase highlights their participation in the viral replication cycle demonstrating their complementary roles.

Hemagglutinin is deeply connected to seasonal influenza outbreaks and pandemics. Alterations in the hemagglutinin protein such as mutations and antigenic drifts or shifts can lead to new influenza strains with pandemic potential. The interaction of hemagglutinin with host immune responses and its capability to change allows the virus to evade immune detection. Neuraminidase inhibitors a class of antiviral drugs target enzymes closely related to hemagglutinin to prevent the spread of infection showcasing hemagglutinin's centrality in both infection and therapeutic intervention strategies.
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一般的な情報

機能

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

配列の類似性

Belongs to the influenza viruses hemagglutinin family.

翻訳後修飾

Palmitoylated.. In natural infection, inactive HA is matured into HA1 and HA2 outside the cell by one or more trypsin-like, arginine-specific endoprotease secreted by the bronchial epithelial cells. One identified protease that may be involved in this process is secreted in lungs by club cells.

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製品プロトコール

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ターゲットの情報

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
See full target information HA
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