Recombinant human/mouse/rat Activin A protein (ab151687)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
製品の詳細
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製品名
Recombinant human/mouse/rat Activin A protein
Activin A タンパク質・ペプチド 製品一覧 -
生理活性
The activity is determined by the ability to induce cytoxicity of MPC-11 cells and is typically less than 2 ng/mL. Corresponding to a specific activity of 5 x 105 units/mg.
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精製度
>= 95 % SDS-PAGE.
Purity is typically >95% as determined by HPLC, Reducing and Non-reducing SDS-PAGE. -
エンドトキシン・レベル
<=1.000 Eu/µg -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MGLECDGKVN ICCKKQFFVS FKDIGWNDWI IAPSGYHANY CEGECPSHIA GTSGSSLSFH STVINHYRMR GHSPFANLKS CCVPTKLRPM SMLYYDDGQN IIKKDIQNMI VEECGCS -
予測される分子量
26 kDa -
領域
311 to 426 -
配列の追加情報
Non-glycosylated homodimer, containing two 117 amino acid chains. Activin A is a homodimer of two beta A chains and is not biologically active until the N terminal propeptide is cleaved from each.
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製品の詳細
Recombinant human Activin A protein
特性
Our Abpromise guarantee covers the use of ab151687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
HPLC
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製品の状態
Lyophilized -
備考
This product shares 100% homology with amino acids 309 - 424 of Rat and Mouse Activin A.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C.
Constituent: 0.02% Trifluoroacetic acid
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water, which can then be further diluted to other aqueous solutions. Reconstituted material should be aliquoted and frozen at -20°C. It is recommended that a carrier protein (0.1% HSA or BSA) is added for long term storage.
関連情報
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別名
- Activin A
- Activin beta A chain
- Activin beta-A chain
see all -
機能
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
画像
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Functional Studies - Recombinant human/mouse/rat Activin A protein (ab151687)Functional analysis of ab151687
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SDS-PAGE - Recombinant human/mouse/rat Activin A protein (ab151687)SDS PAGE analysis of ab151687 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.
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Functional Studies - Recombinant human/mouse/rat Activin A protein (ab151687)Induction of cytotoxicity in MPC-11 cells using ab151687. Assays were carried out in duplicate.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab151687 は 1 報の論文で使用されています。
- Zhoujun Z et al. Transplantation of insulin-producing cells derived from human MSCs to treat diabetes in a non-human primate model. Artif Organs 47:1298-1308 (2023). PubMed: 37032529
