Recombinant Human ZP2 protein (ab159886)
Key features and details
- Expression system: Wheat germ
- Tags: GST tag N-Terminus
- Suitable for: WB, ELISA
製品の詳細
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製品名
Recombinant Human ZP2 protein -
発現系
Wheat germ -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MACRQRGGSWSPSGWFNAGWSTYRSISLFFALVTSGNSIDVSQLVNPAFP GTVTCDEREITVEFPSSPGTKKWHASVVDPLGLDMPNCTYILDPEKLTLR ATYDNCTRRVHGGHQMTIRVMNNSAALRHGAVMYQFFCPAMQVEETQGLS ASTICQKDFMSFSLPRVFSGLADDSKGTKVQMGWSIEVGDGARAKTLTLP EAMKEGFSLLIDNHRMTFHVPFNATGVTHYVQGNSHLYMVSLKLTFISPG QKVIFSSQAICAPDPVTCNATHMTLTIPEFPGKLKSVSFENQNIDVSQLH DNGIDLEATNGMKLHFSKTLLKTKLSEKCLLHQFYLASLKLTFLLRPETV SMVIYPECLCESPVSIVTGELCTQDGFMDVEVYSYQTQPALDLGTLRVGN SSCQPVFEAQSQGLVRFHIPLNGCGTRYKFEDDKVVYENEIHALWTDFPP SKISRDSEFRNDMLLNINVESLTPPVASVKLGPFTLILQSYPDNSYQQPY GENEYPLVRFLRQPIYMEVRVLNRDDPNIKLVLDDCWATSTMDPDSFPQW NVVVDGCAYDLDNYQTTFHPVGSSVTHPDHYQRFDMKAFAFVSEAHVLSS LVYFHCSALICNRLSPDSPLCSVTCPVSSRHRRATGATEAEKMTVSLPGP ILLLSDDSSFRGVGSSDLKASGSSGEKSRSETGEEVGSRGAMDTKGHKTA GDVGSKAVAAVAAFAGVVATLGFIYYLYEKRTVSNH -
領域
1 to 736 -
タグ
GST tag N-Terminus
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特性
Our Abpromise guarantee covers the use of ab159886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
ELISA
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製品の状態
Liquid -
備考
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
関連情報
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別名
- OTTHUMP00000115849
- Processed zona pellucida sperm-binding protein 2
- Zona pellucida glycoprotein 2
see all -
機能
The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor. -
組織特異性
Oocytes. -
配列類似性
Belongs to the ZP domain family. ZPA subfamily.
Contains 1 ZP domain. -
発生段階
Expressed during the 2-week growth phase of oogenesis, prior to ovulation. -
ドメイン
The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida. -
翻訳後修飾
Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
Proteolytically cleaved in the N-terminal part after fertilization, yielding a N-terminal peptide of about 30 kDa which remains covalently attached to the C-terminal peptide via disulfide bond(s). This cleavage may play an important role in the post-fertilization block to polyspermy.
O-glycosylated; contains sulfate-substituted glycans. -
細胞内局在
Secreted > extracellular space > extracellular matrix and Cell membrane. - Information by UniProt
画像
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SDS-PAGE - Recombinant Human ZP2 protein (ab159886)ab159886 on a 12.5% SDS-PAGE stained with Coomassie Blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab159886 は論文での使用が確認できていません。
