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Recombinant human ZAP70 protein (ab60963)

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SDS-PAGE - Recombinant human ZAP70 protein (ab60963)
  • Functional Studies - Recombinant human ZAP70 protein (ab60963)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 95% Densitometry
  • Active: Yes
  • Tags: GST tag N-Terminus
  • Suitable for: Functional Studies

製品の詳細

  • 製品名

    Recombinant human ZAP70 protein
    ZAP70 タンパク質・ペプチド 製品一覧

  • 精製度

    > 95 % Densitometry.
    Affinity purified.

  • 発現系

    Baculovirus infected Sf9 cells

  • アクセッション番号

    P43403

  • タンパク質長

    Full length protein

  • Animal free

    No

  • 由来

    Recombinant

    • 生物種

      Human

    • 予測される分子量

      96 kDa including tags

    • 領域

      1 to 619

    • タグ

      GST tag N-Terminus

特性

Our Abpromise guarantee covers the use of ab60963 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Functional Studies

  • 製品の状態

    Liquid

  • 備考

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol, 0.87% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

関連情報

  • 別名

    • 70 kDa zeta associated protein
    • 70 kDa zeta-associated protein
    • EC 2.7.10.2
    • FLJ17670
    • FLJ17679
    • Selective T cell defect
    • SRK
    • STD
    • Syk related tyrosine kinase
    • Syk-related tyrosine kinase
    • Truncated ZAP kinase
    • Tyrosine protein kinase ZAP70
    • Tyrosine-protein kinase ZAP-70
    • TZK
    • ZAP 70
    • ZAP70
    • ZAP70_HUMAN
    • Zeta chain associated protein kinase 70kD
    • Zeta chain associated protein kinase 70kDa
    • Zeta chain associated protein kinase 70kDa isoform 1
    • Zeta chain associated protein kinase 70kDa isoform 2
    • Zeta chain of T cell receptor associated protein kinase 70
    • Zeta chain TCR associated protein kinase 70kD
    • Zeta chain TCR associated protein kinase 70kDa
    see all

  • 機能

    Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.

  • 組織特異性

    Expressed in T- and natural killer cells.

  • 関連疾患

    Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
    Contains 1 protein kinase domain.
    Contains 2 SH2 domains.

  • ドメイン

    The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH.

  • 翻訳後修飾

    Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity.

  • 細胞内局在

    Cytoplasm. Cell membrane. After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes.
  • Information by UniProt

画像

  • SDS-PAGE - Recombinant human ZAP70 protein (ab60963)
    SDS-PAGE - Recombinant human ZAP70 protein (ab60963)
    SDS PAGE analysis of ab60963
  • Functional Studies - Recombinant human ZAP70 protein (ab60963)
    Functional Studies - Recombinant human ZAP70 protein (ab60963)
    The specific activity of ZAP70 (ab60963) was determined to be 129 nmol/min/mg as per activity assay protocol

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab60963 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab60963 は論文での使用が確認できていません。

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