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AB196076

Recombinant human Yes1 protein (GST tag N-Terminus)

Recombinant human Yes1 protein (GST tag N-Terminus)

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Recombinant human Yes1 protein (GST tag N-Terminus) is a Human Full Length protein, in the 2 to 543 aa range, expressed in Baculovirus infected Sf9 cells, with >70%, suitable for FuncS.

別名を表示する

YES, YES1, Tyrosine-protein kinase Yes, Proto-oncogene c-Yes, p61-Yes

2 Images
Functional Studies - Recombinant human Yes1 protein (GST tag N-Terminus) (AB196076)
  • FuncS

Unknown

Functional Studies - Recombinant human Yes1 protein (GST tag N-Terminus) (AB196076)

Specific activity of ab196076 was determined to be 200 pmol/min/μg in a kinase assay using Poly-(Glu4 : Tyr)-biotin as substrate.

SDS-PAGE - Recombinant human Yes1 protein (GST tag N-Terminus) (AB196076)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Yes1 protein (GST tag N-Terminus) (AB196076)

SDS-PAGE analysis of ab196076.

Key facts

精製度

>70% SDS-PAGE

発現系

Baculovirus infected Sf9 cells

タグ

GST tag N-Terminus

アプリケーション

FuncS

applications

生物活性

Yes

生物学的活性

Assay was done in Kinase buffer containing 0.2 mM DTT using Poly-(Glu4:Tyr)-biotin substrate (0.2 mg/ml) and 20 μM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred was calculated.

アクセッション番号

P07947

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"sequence":"GCIKSKENKSPAIKYRPENTPEPVSTSVSHYGAEPTTVSPCPSSSAKGTAVNFSSLSMTPFGGSSGVTPFGGASSSFSVVPSSYPAGLTGGVTIFVALYDYEARTTEDLSFKKGERFQIINNTEGDWWEARSIATGKNGYIPSNYVAPADSIQAEEWYFGKMGRKDAERLLLNPGNQRGIFLVRESETTKGAYSLSIRDWDEIRGDNVKHYKIRKLDNGGYYITTRAQFDTLQKLVKHYTEHADGLCHKLTTVCPTVKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTKVAIKTLKPGTMMPEAFLQEAQIMKKLRHDKLVPLYAVVSEEPIYIVTEFMSKGSLLDFLKEGDGKYLKLPQLVDMAAQIADGMAYIERMNYIHRDLRAANILVGENLVCKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILQTELVTKGRVPYPGMVNREVLEQVERGYRMPCPQGCPESLHELMNLCWKKDPDERPTFEYIQSFLEDYFTATEPQYQPGENL","proteinLength":"Full Length","predictedMolecularWeight":"91 kDa","actualMolecularWeight":null,"aminoAcidEnd":543,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"P07947","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

出荷温度
Dry Ice
短期保存温度
-80°C
長期保存温度
-80°C
保管に関する情報
Avoid freeze / thaw cycle
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Yes1 protein also known as p61-Yes is a member of the Src family kinases (SFKs) and has a molecular mass of approximately 61 kDa. This non-receptor tyrosine kinase engages in phosphorylation of tyrosine residues playing a significant role in cell signaling. It is widely expressed in many tissues including the brain heart and lungs suggesting its involvement in varied physiological processes.
Biological function summary

The Yes1 protein participates in cellular processes such as cell growth survival and differentiation. It contributes to signaling cascades that regulate these functions often working as part of protein complexes. Its ability to interact with various signaling molecules positions it as an important player in the control of cell behavior particularly in response to extracellular stimuli.

Pathways

The Yes1 protein integrates into key signaling routes like the epidermal growth factor receptor (EGFR) signaling pathway and the integrin signaling pathway. Within these pathways Yes1 interacts with proteins like c-Src and Fyn which are other members of the Src family. These pathways are vital in mediating cellular communication for growth and attachment highlighting Yes1's role in transmitting signals that prompt cellular responses.

Research connects Yes1 protein to several types of cancer such as colon cancer and breast cancer. The dysregulation of Yes1 along with associated proteins like c-Myc may lead to uncontrolled cell proliferation typical of tumorigenesis. Understanding these interactions offers insights into potential therapeutic targets as altering Yes1 activity might influence cancer progression or treatment outcomes.
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製品の性状

製品の状態

Liquid

補足情報

Affinity purified.

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一般的な情報

機能

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity (PubMed : 26979622).

配列の類似性

Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.

翻訳後修飾

Phosphorylated (PubMed:11901164). Phosphorylation by CSK on the C-terminal tail maintains the enzyme in an inactive state. Autophosphorylation at Tyr-426 maintains enzyme activity by blocking CSK-mediated inhibition.. Palmitoylation at Cys-3 promotes membrane localization.

細胞内局在性

Cytoskeleton

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製品プロトコール

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ターゲットの情報

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGFR, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis. Catalyzes phosphorylation of organic cation transporter OCT2 which induces its transport activity (PubMed : 26979622).
See full target information YES1
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組合せ製品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB305327

Anti-Yes antibody [1/Yes]

Recombinant|Trial Size

Western blot - Anti-Yes antibody [1/Yes] (AB305327)

  • 0
  • 0
Primary Antibodies

AB192890

Anti-LC3B antibody [EPR18709] - Autophagosome Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

  • 5
  • 19
Proteins & Peptides

AB42619

Recombinant human JAK2 protein (His tag N-Terminus)

Functional Studies - Recombinant human JAK2 protein (His tag N-Terminus) (AB42619)

  • 0
  • 0

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