Recombinant human Wnt9b protein (Active) (ab245815)
Key features and details
- Expression system: CHO cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human Wnt9b protein (Active)
Wnt9b タンパク質・ペプチド 製品一覧 -
生理活性
Determined by its ability to induce alkaline phosphatase production by CCL-226 cells.
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精製度
>= 95 % SDS-PAGE.
At least 95% by HPLC analysis. -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
CHO cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
SYFGLTGREVLTPFPGLGTAAAPAQGGAHLKQCDLLKLSRRQKQLCRREP GLAETLRDAAHLGLLECQFQFRHERWNCSLEGRMGLLKRGFKETAFLYAV SSAALTHTLARACSAGRMERCTCDDSPGLESRQAWQWGVCGDNLKYSTKF LSNFLGSKRGNKDLRARADAHNTHVGIKAVKSGLRTTCKCHGVSGSCAVR TCWKQLSPFRETGQVLKLRYDSAVKVSSATNEALGRLELWAPARQGSLTK GLAPRSGDLVYMEDSPSFCRPSKYSPGTAGRVCSREASCSSLCCGRGYDT QSRLVAFSCHCQVQWCCYVECQQCVQEELVYTCKH -
予測される分子量
37 kDa -
領域
23 to 357 -
配列の追加情報
Full-length mature chain lacking the signal peptide.
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特性
Our Abpromise guarantee covers the use of ab245815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
HPLC
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
備考
Due to glycosylation, ab245815 migrates at an apparent molecular weight of approximately 49-54 kDa by SDS-PAGE analysis under non-reducing conditions.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.12% Tris, 0.56% Potassium chloride, 1.45% Sodium chloride, 0.375% CHAPS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Centrifuge vial prior to opening. Reconstitute in water to 0.1-0.5 mg/ml. Do not vortex. Store at 4°C for 1 week, or prepare for extended storage. Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA). Store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
関連情報
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別名
- MGC124412
- Protein Wnt 9b precursor
- Protein Wnt-14b
see all -
関連性
Wnt9b is a member of the WNT family and is a ligand for members of the frizzled family of seven transmembrane receptors. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. Wnt9b is expressed in the inductive epithelia and is essential for the development of mesonephric and metanephric tubules and caudal extension of the Müllerian duct. Wnt9b plays a central role in the regulation of mesenchymal to epithelial transitions underlying organogenesis of the mammalian urogenital system. The gene is clustered with WNT3, another family member, in the chromosome 17q21 region. -
細胞内局在
Secreted: extracellular space: extracellular matrix.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab245815 は論文での使用が確認できていません。