Recombinant human WISP2 protein (ab50040)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
製品の詳細
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製品名
Recombinant human WISP2 protein
WISP2 タンパク質・ペプチド 製品一覧 -
生理活性
Biological Activity :The ED50 was determined by its ability to inhibit IGF-II induced proliferation of MCF-7 is between 10-20 ng/ml in the presence of 15 ng/ml of human IGF-II.
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精製度
> 95 % SDS-PAGE.
Endotoxin level is less than 0.1 ng per µg (1EU/µg). -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MQLCPTPCTC PWPPPRCPLG VPLVLDGCGC CRVCARRLGE PCDQLHVCDA SQGLVCQPGA GPGGRGALCL LAEDDSSCEV NGRLYREGET FQPHCSIRCR CEDGGFTCVP LCSEDVRLPS WDCPHPRRVE VLGKCCPEWV CGQGGGLGTQ PLPAQGPQFS GLVSSLPPGV PCPEWSTAWG PCSTTCGLGM ATRVSNQNRF CRLETQRRLC LSRPCPPSRG RSPQNSAF
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab50040 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Western blot
SDS-PAGE
Functional Studies
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Note: Do not raise to neutral pH. Do not vortex. This solution can be stored at 2-8oC for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.
関連情報
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別名
- CCN family member 5
- CCN5
- Connective tissue growth factor like protein
see all -
機能
May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production. -
組織特異性
Expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart. -
配列類似性
Belongs to the CCN family.
Contains 1 IGFBP N-terminal domain.
Contains 1 TSP type-1 domain.
Contains 1 VWFC domain. -
細胞内局在
Secreted. - Information by UniProt
画像
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Western blot - Recombinant human WISP2 protein (ab50040)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab50040 は 1 報の論文で使用されています。
- Grünberg JR et al. Overexpressing the novel autocrine/endocrine adipokine WISP2 induces hyperplasia of the heart, white and brown adipose tissues and prevents insulin resistance. Sci Rep 7:43515 (2017). PubMed: 28240264
