AB269132

Recombinant Human WDR5 protein (His tag)

Name: Recombinant Human WDR5 protein (His tag) (ab269132) | Abcam
Brand: Abcam Limited
SKU: AB269132
Price: 49000 JPY
Availability: InStock

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Recombinant Human WDR5 protein (His tag) is a Human Full Length protein, in the 1 to 334 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

別名を表示する

BIG3, WDR5, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein

1 Images

SDS-PAGE - Recombinant Human WDR5 protein (His tag) (AB269132)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human WDR5 protein (His tag) (AB269132)

Recombinant full-length human WDR5 protein (ab269132) resolved on a SDS-PAGE gel.

Key facts

精製度

>85% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE

applications

生物活性

アクセッション番号

P61964

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7 Preservative: 1.02% Imidazole Constituents: 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride, 0.82% Sodium phosphate, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.002% PMSF

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"linker":null,"sequence":"MATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":334,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P61964","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

WDR5 also known as WD repeat domain 5 is an important component in the regulation of gene expression. It has a molecular mass of approximately 36 kDa. This protein is widely expressed across various tissues with significant expression in the nucleus. WDR5 primarily functions as a scaffolding protein facilitating the assembly of multiprotein complexes that modify chromatin structure and function. Its involvement in chromatin regulation highlights its mechanical role in modulating access to DNA for transcriptional machinery.

Biological function summary

WDR5 plays a significant role in epigenetic regulation by being part of the histone methyltransferase complex associated with MLL (Mixed Lineage Leukemia). This complex is responsible for the methylation of histone H3 at lysine 4 (H3K4) an essential modification for transcriptional activation. WDR5's interaction with MLL complex components influences gene expression importantly during development and cellular differentiation. Its presence in the MLL complex connects it to pathways that govern cell cycle regulation and stem cell maintenance.

Pathways

WDR5 functions within key pathways such as the chromatin modification pathway and the WNT signaling pathway. In these settings WDR5 interacts with proteins like MLL and BRG1 which contribute to its role in gene regulation and chromatin remodeling. Through these interactions WDR5 modulates the transcription of target genes impacting cellular processes like proliferation and differentiation.

WDR5 has shown involvement in certain cancers and neurodevelopmental disorders. Its dysregulation is linked to leukemia specifically in cases where MLL rearrangements alter its normal function. Additionally aberrant expression of WDR5 may influence neurodevelopmental disorders though its precise role requires more research. In the context of leukemia WDR5's interaction with proteins such as DOT1L and MEN1 supports the proliferation of leukemic cells. Understanding the involvement of WDR5 in these conditions can offer insights into therapeutic targets for disease intervention.

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製品の性状

製品の状態

Liquid

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一般的な情報

機能

Contributes to histone modification (PubMed : 16600877, PubMed : 16829960, PubMed : 19103755, PubMed : 19131338, PubMed : 19556245, PubMed : 20018852). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed : 16829960). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 18840606). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed : 19103755, PubMed : 20018852). May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).

配列の類似性

Belongs to the WD repeat WDR5/wds family.

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information WDR5

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Abcam product promise

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Recombinant Human WDR5 protein (His tag)