AB98079

Recombinant Human WDR5 protein (His tag N-Terminus)

Name: Recombinant Human WDR5 protein (ab98079) | Abcam
Brand: Abcam Limited
SKU: AB98079
Price: 68000 JPY
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Recombinant Human WDR5 protein (His tag N-Terminus) is a Human Full Length protein, in the 1 to 334 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

BIG3, WDR5, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein

1 Images

SDS-PAGE - Recombinant Human WDR5 protein (His tag N-Terminus) (AB98079)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human WDR5 protein (His tag N-Terminus) (AB98079)

15% SDS-PAGE analysis of 3µg ab98079

Key facts

精製度

>95% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE, Mass Spec

applications

生物活性

アクセッション番号

P61964

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

style

left-column

配列情報

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMATEEKKPETEAARAQPTPSSSATQSKPTPVKPNYALKFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLKGHSNYVFCCNFNPQSNLIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLKTYTGHKNEKYCIFANFSVTGGKWIVSGSEDNLVYIWNLQTKEIVQKLQGHTDVVISTACHPTENIIASAALENDKTIKLWKSDC","proteinLength":"Full Length","predictedMolecularWeight":"38.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":334,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P61964","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

style

left-column

出荷温度及び保存条件

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

style

left-column

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

WDR5 also known as WD repeat domain 5 is an important component in the regulation of gene expression. It has a molecular mass of approximately 36 kDa. This protein is widely expressed across various tissues with significant expression in the nucleus. WDR5 primarily functions as a scaffolding protein facilitating the assembly of multiprotein complexes that modify chromatin structure and function. Its involvement in chromatin regulation highlights its mechanical role in modulating access to DNA for transcriptional machinery.

Biological function summary

WDR5 plays a significant role in epigenetic regulation by being part of the histone methyltransferase complex associated with MLL (Mixed Lineage Leukemia). This complex is responsible for the methylation of histone H3 at lysine 4 (H3K4) an essential modification for transcriptional activation. WDR5's interaction with MLL complex components influences gene expression importantly during development and cellular differentiation. Its presence in the MLL complex connects it to pathways that govern cell cycle regulation and stem cell maintenance.

Pathways

WDR5 functions within key pathways such as the chromatin modification pathway and the WNT signaling pathway. In these settings WDR5 interacts with proteins like MLL and BRG1 which contribute to its role in gene regulation and chromatin remodeling. Through these interactions WDR5 modulates the transcription of target genes impacting cellular processes like proliferation and differentiation.

WDR5 has shown involvement in certain cancers and neurodevelopmental disorders. Its dysregulation is linked to leukemia specifically in cases where MLL rearrangements alter its normal function. Additionally aberrant expression of WDR5 may influence neurodevelopmental disorders though its precise role requires more research. In the context of leukemia WDR5's interaction with proteins such as DOT1L and MEN1 supports the proliferation of leukemic cells. Understanding the involvement of WDR5 in these conditions can offer insights into therapeutic targets for disease intervention.

style

left-column

製品の性状

製品の状態

Liquid

補足情報

ab98079 was purified using conventional chromatography techniques.

style

left-column

一般的な情報

機能

Contributes to histone modification (PubMed : 16600877, PubMed : 16829960, PubMed : 19103755, PubMed : 19131338, PubMed : 19556245, PubMed : 20018852). May position the N-terminus of histone H3 for efficient trimethylation at 'Lys-4' (PubMed : 16829960). As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3 (PubMed : 19556245). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed : 18840606). As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed : 19103755, PubMed : 20018852). May regulate osteoblasts differentiation (By similarity). In association with RBBP5 and ASH2L, stimulates the histone methyltransferase activities of KMT2A, KMT2B, KMT2C, KMT2D, SETD1A and SETD1B (PubMed : 21220120, PubMed : 22266653).

配列の類似性

Belongs to the WD repeat WDR5/wds family.

細胞内局在性

Nucleus

style

left-column

製品プロトコール

Visit the General protocols
Visit the Troubleshooting

style

left-column

ターゲットの情報

See full target information WDR5

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 39:110836 PubMed35584667

2022

H3-K27M-mutant nucleosomes interact with MLL1 to shape the glioma epigenetic landscape.

Applications

Unspecified application

Species

Unspecified reactive species

Noa Furth,Danielle Algranati,Bareket Dassa,Olga Beresh,Vadim Fedyuk,Natasha Morris,Lawryn H Kasper,Dan Jones,Michelle Monje,Suzanne J Baker,Efrat Shema

mBio 12:e0343121 PubMed34933446

2021

GRWD1-WDR5-MLL2 Epigenetic Complex Mediates H3K4me3 Mark and Is Essential for Kaposi's Sarcoma-Associated Herpesvirus-Induced Cellular Transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Wei,Songjian Lu,Lifan Liang,Xian Wang,Wan Li,Tingting Li,Luping Chen,Enguo Ju,Xinquan Zhang,Zhao Lai,Yufei Huang,Xinghua Lu,Shou-Jiang Gao

View all publications

style

left-column

style

left-column

style

right-column

Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Recombinant Human WDR5 protein (His tag N-Terminus)