AB125555

Recombinant human VRK1 protein

Name: Recombinant human VRK1 protein (ab125555) | Abcam
Brand: Abcam Limited
SKU: AB125555
Price: 69000 JPY
Availability: InStock

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(1 Publication)

Recombinant human VRK1 protein is a Human Full Length protein, in the 1 to 396 aa range, expressed in Baculovirus infected Sf9 cells, with >95%, suitable for SDS-PAGE, WB, FuncS.

別名を表示する

Serine/threonine-protein kinase VRK1, Vaccinia-related kinase 1, VRK1

2 Images

Functional Studies - Recombinant human VRK1 protein (AB125555)

FuncS

Unknown

Functional Studies - Recombinant human VRK1 protein (AB125555)

Sample Kinase Assay showing the Specific Activity of ab125555 to be 2 nmol/min/mg.

SDS-PAGE - Recombinant human VRK1 protein (AB125555)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant human VRK1 protein (AB125555)

SDS-PAGE analysis of ab125555.

Key facts

精製度

>95% Densitometry

発現系

Baculovirus infected Sf9 cells

アプリケーション

WB, SDS-PAGE, FuncS

applications

生物活性

Yes

生物学的活性

The specific activity of ab125555 was determined to be 2 nmol/min/mg.

アクセッション番号

Q99986

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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製品の詳細

ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity

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配列情報

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":"71 kDa","actualMolecularWeight":null,"aminoAcidEnd":396,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"Q99986","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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製品の性状

製品の状態

Liquid

補足情報

Purity was determined to be >95% by densitometry.

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一般的な情報

機能

Serine/threonine kinase involved in the regulation of key cellular processes including the cell cycle, nuclear condensation, transcription regulation, and DNA damage response (PubMed : 14645249, PubMed : 18617507, PubMed : 19103756, PubMed : 33076429). Controls chromatin organization and remodeling by mediating phosphorylation of histone H3 on 'Thr-4' and histone H2AX (H2aXT4ph) (PubMed : 31527692, PubMed : 37179361). It also phosphorylates KAT5 in response to DNA damage, promoting KAT5 association with chromatin and histone acetyltransferase activity (PubMed : 33076429). Is involved in the regulation of cell cycle progression of neural progenitors, and is required for proper cortical neuronal migration (By similarity). Is involved in neurite elongation and branching in motor neurons, and has an essential role in Cajal bodies assembly, acting through COIL phosphorylation and the control of coilin degradation (PubMed : 21920476, PubMed : 31090908, PubMed : 31527692). Involved in Golgi disassembly during the cell cycle : following phosphorylation by PLK3 during mitosis, it is required to induce Golgi fragmentation (PubMed : 19103756). Phosphorylates BANF1 : disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm (PubMed : 16495336). Phosphorylates TP53BP1 and p53/TP53 on 'Thr-18', preventing the interaction between p53/TP53 and MDM2 (PubMed : 10951572, PubMed : 31527692). Phosphorylates ATF2 which activates its transcriptional activity (PubMed : 15105425). Phosphorylates JUN (PubMed : 31527692).

配列の類似性

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.

翻訳後修飾

Autophosphorylated at various serine and threonine residues (PubMed:11883897, PubMed:14645249, PubMed:19103756, PubMed:21543316). Autophosphorylation does not impair its ability to phosphorylate p53/TP53 (PubMed:11883897). Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis (PubMed:19103756).

細胞内局在性

Nucleus

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information VRK1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 12:11210 PubMed35778595

2022

Dissecting the roles of Haspin and VRK1 in histone H3 phosphorylation during mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tyrell N Cartwright,Rebecca J Harris,Stephanie K Meyer,Aye M Mon,Nikolaus A Watson,Cheryl Tan,Agathe Marcelot,Fangwei Wang,Sophie Zinn-Justin,Paula Traktman,Jonathan M G Higgins

View all publications

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Abcam product promise

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Recombinant human VRK1 protein