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Recombinant Human Vimentin protein (Tagged-His Tag) (ab84704)

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SDS-PAGE - Recombinant Human Vimentin protein (ab84704)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, WB

    製品の詳細

    • 製品名

      Recombinant Human Vimentin protein (Tagged-His Tag)
      Vimentin タンパク質・ペプチド 製品一覧

    • 精製度

      > 95 % SDS-PAGE.
      Purity >95% as determined by densitometry.

    • 発現系

      Escherichia coli

    • アクセッション番号

      P08670

    • タンパク質長

      Full length protein

    • Animal free

      No

    • 由来

      Recombinant

      • 生物種

        Human

      • 配列

        MSTRSVSSSS YRRMFGGPGT ASRPSSSRSY VTTSTRTYSL GSALRPSTSR SLYASSPGGV YATRSSAVRL RSSVPGVRLL QDSVDFSLAD AINTEFKNTR TNEKVELQEL NDRFANYIDK VRFLEQQNKI LLAELEQLKG QGKSRLGDLY EEEMRELRRQ VDQLTNDKAR VEVERDNLAE DIMRLREKLQ EEMLQREEAE NTLQSFRQDV DNASLARLDL ERKVESLQEE IAFLKKLHEE EIQELQAQIQ EQHVQIDVDV SKPDLTAALR DVRQQYESVA AKNLQEAEEW YKSKFADLSE AANRNNDALR QAKQESTEYR RQVQSLTCEV DALKGTNESL ERQMREMEEN FAVEAANYQD TIGRLQDEIQ NMKEEMARHL REYQDLLNVK MALDIEIATY RKLLEGEESR ISLPLPNFSS LNLRETNLDS LPLVDTHSKR TLLIKTVETR DGQVINETSQ HHDDLE

      • 予測される分子量

        60 kDa including tags

      • 領域

        1 to 466

      • タグ

        His tag N-Terminus

    特性

    Our Abpromise guarantee covers the use of ab84704 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • アプリケーション

      SDS-PAGE

      Western blot

    • 製品の状態

      Liquid
    • Concentration information loading...

    前処理および保存

    • 保存方法および安定性

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.00
      Preservative: 1.02% Imidazole
      Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.0308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride

    関連情報

    • 別名

      • CTRCT30
      • Epididymis luminal protein 113
      • FLJ36605
      • HEL113
      • VIM
      • VIME_HUMAN
      • Vimentin
      see all

    • 機能

      Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
      Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

    • 組織特異性

      Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

    • 関連疾患

      Cataract 30

    • 配列類似性

      Belongs to the intermediate filament family.

    • ドメイン

      The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

    • 翻訳後修飾

      Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
      O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

    • 細胞内局在

      Cytoplasm.
    • Information by UniProt

    画像

    • SDS-PAGE - Recombinant Human Vimentin protein (ab84704)
      SDS-PAGE - Recombinant Human Vimentin protein (ab84704)
      SDS-PAGE of ab84704 using 3ug Vimentin protein. Detected molecular weight approximately 60 kDa.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab84704 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab84704 は 1 報の論文で使用されています。

    • Kong Q  et al. Identification and characterization of an oocyte factor required for porcine nuclear reprogramming. J Biol Chem 289:6960-8 (2014). PubMed: 24474691

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