Recombinant Human VEGFC protein (Active) (ab283476)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Tags: Glycine tag N-Terminus
- Suitable for: Biological Activity, SDS-PAGE, HPLC
製品の詳細
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製品名
Recombinant Human VEGFC protein (Active)
VEGFC タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by dose dependent induction of NFAT-mediatedgene activation in HEK293 overexpressing human VEGFR2/KDR and NFAT-luciferase reporter cell line.
ED50 is ≤ 10.4 ng/ml, corresponding to a specific activity of 9.6 x 104 units/mg.
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精製度
>= 95 % HPLC. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
AHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGVATNTFFKPPCVSVYR CGGCCNSEGLQCMNTSTSYLSKTLFEITVPLSQGPKPVTISFANHTSCRC MSKLDVYRQVHSIIRR -
予測される分子量
46 kDa -
領域
112 to 227 -
タグ
Glycine tag N-Terminus -
配列の追加情報
Full length mature chain, lacking the signal peptide, N-terminal Glycine.
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特性
Our Abpromise guarantee covers the use of ab283476 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Biological Activity
SDS-PAGE
HPLC
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Store lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- Flt 4L
- Flt4 ligand
- FLT4 ligand DHM
see all -
機能
Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. -
組織特異性
Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte. -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
翻訳後修飾
Undergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions. -
細胞内局在
Secreted. - Information by UniProt
画像
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Biological Activity - Recombinant Human VEGFC protein (Active) (ab283476)
Fully biologically active determined by dose dependent induction of NFAT-mediatedgene activation in HEK293 overexpressing human VEGFR2/KDR and NFAT-luciferase reporter cell line.
ED50 is ≤ 10.4 ng/ml, corresponding to a specific activity of 9.6 x 104 units/mg.
Cell based assay testing is performed on the first lot of the protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot GR3427660-2.
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SDS-PAGE - Human VEGFC protein (ab283476)SDS-PAGE analysis of ab283476.
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HPLC - Recombinant Human VEGFC protein (ab283476)HPLC analysis of ab283476.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab283476 は論文での使用が確認できていません。
