AB55566

Recombinant Human VEGFA protein

Name: Recombinant Human VEGFA protein (ab55566) | Abcam
Brand: Abcam Limited
SKU: AB55566
Rating: 5 (1 reviews)

(1 Review)

(6 Publications)

Recombinant Human VEGFA protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

別名を表示する

VEGF, VEGFA, L-VEGF, Vascular permeability factor, VPF

1 Images

SDS-PAGE - Recombinant Human VEGFA protein (AB55566)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human VEGFA protein (AB55566)

ab55566 on SDS-PAGE

Key facts

精製度

>95% SDS-PAGE

発現系

Escherichia coli

アプリケーション

SDS-PAGE, WB

applications

生物活性

アクセッション番号

P15692

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 0.242% Tris

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab55566 can be used as a WB positive control in conjunction with ab68334.</p>" } } }

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配列情報

[{"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":"23.6 kDa","aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P15692","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.

Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.

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製品の性状

製品の状態

Liquid

補足情報

Proprietary purification method

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一般的な情報

機能

N-VEGF. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A (PubMed : 35455969). Involved in protecting cells from hypoxia-mediated cell death (By similarity).. VEGFA. Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth (PubMed : 34530889). Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (PubMed : 17446437).. Isoform VEGF165B. Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

配列の類似性

Belongs to the PDGF/VEGF growth factor family.

翻訳後修飾

Vascular endothelial growth factor A, long form. Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information VEGFA

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文献 (6)

Recent publications for all applications. Explore the full list and refine your search

Life (Basel, Switzerland) 13: PubMed38004368

2023

Mathematical Modeling of Non-Small-Cell Lung Cancer Biology through the Experimental Data on Cell Composition and Growth of Patient-Derived Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Rushan Sulimanov,Konstantin Koshelev,Vladimir Makarov,Alexandre Mezentsev,Mikhail Durymanov,Lilian Ismail,Komal Zahid,Yegor Rumyantsev,Ilya Laskov

Cell death & disease 11:1016 PubMed33247124

2020

Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Liu,Aisong Guo,Yuanyuan Tu,Wendie Li,Lele Li,Wangrui Liu,Yuanyuan Ju,Yamei Zhou,Aimin Sang,Manhui Zhu

Journal of medicinal food : PubMed32614624

2020

Angiogenesis is Differentially Modulated by Anthocyanin and Phenolic Acid Extracts from Wild Blueberry () Through PI3K Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Panagiotis Tsakiroglou,James Weber,Sharon Ashworth,Cristian Del Bo',Dorothy Klimis-Zacas

Cell death & disease 9:906 PubMed30185783

2018

Polyphyllin I suppresses the formation of vasculogenic mimicry via Twist1/VE-cadherin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Xiao,Weilong Zhong,Jianmin Zhao,Baoxin Qian,Huijuan Liu,Shuang Chen,Kailiang Qiao,Yueyang Lei,Shumin Zong,Hongzhi Wang,Yuan Liang,Heng Zhang,Jing Meng,Honggang Zhou,Tao Sun,Yanrong Liu,Cheng Yang

PloS one 10:e0144823 PubMed26657744

2015

Adipose-Derived Stromal Vascular Fraction Cell Effects on a Rodent Model of Thin Endometrium.

Applications

Species

Unspecified reactive species

Robert K Hunter,Chris D Nevitt,Jeremy T Gaskins,Bradley B Keller,Henry C L Bohler,Amanda J LeBlanc

Cardiovascular research 93:111-9 PubMed22002459

2011

ARNO regulates VEGF-dependent tissue responses by stabilizing endothelial VEGFR-2 surface expression.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna K Mannell,Joachim Pircher,Daniel I Chaudhry,Stefan K C Alig,Elisabeth G Koch,Ramona Mettler,Ulrich Pohl,Florian Krötz

View all publications

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Recombinant Human VEGFA protein