Recombinant human VEGF 165A protein (Active) (Biotin) (ab168684)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Achieve higher consistency and quality standards with a premium grade bioactive protein
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品の詳細
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製品名
Recombinant human VEGF 165A protein (Active) (Biotin)
VEGF 165A タンパク質・ペプチド 製品一覧 -
生理活性
The bio-activity was determined by dose-dependent stimulation of the proliferation of HUVEC cells.
ED 50: 2X105 Unit/mg.
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精製度
> 95 % SDS-PAGE.
Purified by ion exchnage chromatography + Label -
エンドトキシン・レベル
< 1.000 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Protein fragment -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPS CVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHN KCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQ LELNERTCRCDKPRR -
予測される分子量
19 kDa -
領域
27 to 191
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標識
Biotin
関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab168684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Lyophilized -
備考
A standard biotin reagent (13.5 angstroms) is used in this product.
Biotin:Protein ratio The biotin to protein ratio is 3-5 as determined by the HABA assay.
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 100 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.
関連情報
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別名
- glioma-derived endothelial cell mitogen
- MGC70609
- MVCD1
see all -
機能
Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. -
組織特異性
Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. -
関連疾患
Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. -
配列類似性
Belongs to the PDGF/VEGF growth factor family. -
細胞内局在
Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. - Information by UniProt
画像
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Functional Studies - Recombinant human VEGF 165A protein (Biotin) (ab168684)
Immobilized human VEGFR1/R2, Fc tag at 2μg/mL (100 μL/well) binds ab168684.
Linear range: 0.39-3.1 ng/mL (QC tested).
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Functional Studies - Recombinant human VEGF 165A protein (Biotin) (ab168684)Immobilized Human Neuropilin-1, His Tag at 2 μg/mL (100 μL/well) binds ab168684.
Linear range: 5-40 ng/mL (Routinely tested).
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Functional Studies - Recombinant human VEGF 165A protein (Biotin) (ab168684)Immobilized Anti-VEGF MAb,Human IgG1 (Avastin) at 5μg/mL (100 μL/well) binds ab168684.
Linear range: 0.24-1.95 ng/mL (Routinely tested).
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SDS-PAGE - Recombinant human VEGF 165A protein (Biotin) (ab168684)Reduced (Lane 1) and non-reduced (Lane 2) ab168684 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein >95%.
As a result of glycosylation, the protein migrates as 25-30 kDa (monomer) under reducing condition, and 43-50 kDa under non-reducing condition.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab168684 は論文での使用が確認できていません。