Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
- High batch-to-batch consistency
- Optimal bioactivity
- Guaranteed identical to human native proteins
- >95% purity
- Ultra-low endotoxin levels: <0.005 Eu/µg
- Carrier and tag free
製品名Recombinant human VEGF 165A protein (Active) (Biotin)
VEGF 165A タンパク質・ペプチド 製品一覧
The bio-activity was determined by dose-dependent stimulation of the proliferation of HUVEC cells.
ED 50: 2X105 Unit/mg.
精製度> 95 % SDS-PAGE.
Purified by ion exchnage chromatography + Label
エンドトキシン・レベル< 1.000 Eu/µg
発現系HEK 293 cells
配列APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPS CVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHN KCECRPKKDRARQENPCGPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQ LELNERTCRCDKPRR
領域27 to 191
Our Abpromise guarantee covers the use of ab168684 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
A standard biotin reagent (13.5 angstroms) is used in this product.
Biotin:Protein ratio The biotin to protein ratio is 3-5 as determined by the HABA assay.
Concentration information loading...
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: PBS, 5% Trehalose
Lyophilized from 0.22 µm filtered solution.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
再構成It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 100 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing.
- glioma-derived endothelial cell mitogen
機能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
組織特異性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
関連疾患Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
配列類似性Belongs to the PDGF/VEGF growth factor family.
細胞内局在Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
- Information by UniProt
Immobilized human VEGFR1/R2, Fc tag at 2μg/mL (100 μL/well) binds ab168684.
Linear range: 0.39-3.1 ng/mL (QC tested).
Immobilized Human Neuropilin-1, His Tag at 2 μg/mL (100 μL/well) binds ab168684.
Linear range: 5-40 ng/mL (Routinely tested).
Immobilized Anti-VEGF MAb,Human IgG1 (Avastin) at 5μg/mL (100 μL/well) binds ab168684.
Linear range: 0.24-1.95 ng/mL (Routinely tested).
Reduced (Lane 1) and non-reduced (Lane 2) ab168684 on SDS-PAGE, stained overnight with Coomassie Blue.
Purity of protein >95%.
As a result of glycosylation, the protein migrates as 25-30 kDa (monomer) under reducing condition, and 43-50 kDa under non-reducing condition.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.