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AB288799

Recombinant Human VCAM1 protein (Active)

Recombinant Human VCAM1 protein (Active)

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Recombinant Human VCAM1 protein (Active) is a Human Fragment protein, in the 25 to 698 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for FuncS.

別名を表示する

CD106, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, VCAM1

4 Images
Functional Studies - Recombinant Human VCAM1 protein (Active) (AB288799)
  • FuncS

Lab

Functional Studies - Recombinant Human VCAM1 protein (Active) (AB288799)

Addition of PMA-induced U937 cells to human VCAM-1 coated plates (1.25 μg/mL, 100 μL/well) led to 74.12% cell adherence specifically. Statistical analysis was performed using two-tailed unpaired t-test, ***=p<0.0002.

Mass Spectrometry - Recombinant Human VCAM1 protein (Active) (AB288799)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Human VCAM1 protein (Active) (AB288799)

Mass determination by ESI-TOF.

Predicted MW is 74284.60 Da. (+/- 10 Da by ESI-TOF). Observed MW is 74291.89 Da.

SDS-PAGE - Recombinant Human VCAM1 protein (Active) (AB288799)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human VCAM1 protein (Active) (AB288799)

SDS-PAGE analysis of ab288799

HPLC - Recombinant Human VCAM1 protein (Active) (AB288799)
  • HPLC

Supplier Data

HPLC - Recombinant Human VCAM1 protein (Active) (AB288799)

HPLC analysis of ab288799

Key facts

精製度

>95% HPLC

エンドトキシンレベル

<0.005 EU/µg

発現系

HEK 293 cells

タグ

Tag free

アプリケーション

FuncS

applications

生物活性

Yes

生物学的活性

Addition of PMA-induced U937 cells to human VCAM-1 coated plates (1.25 μg/mL, 100 μL/well) led to 74.12% cell adherence specifically. Statistical analysis was performed using two-tailed unpaired t-test, ***=p<0.0002.

アクセッション番号

P19320

アニマルフリー

No

キャリアフリー

No

種

Human

再構成

Reconstitute in PBS

バッファー組成

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

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Proteins & Peptides

AB283423

Recombinant Human Fibronectin protein

Mass Spectrometry - Recombinant Human Fibronectin protein (AB283423)

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Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

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Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

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Assay Kits

AB223591

Human VCAM1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human VCAM1 ELISA Kit (AB223591)

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Proteins & Peptides

AB283898

Recombinant Human CXCL4 protein (Active)

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Mass Spectrometry - Recombinant Human CXCL4 protein (Active) (AB283898)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"FKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPE","proteinLength":"Fragment","predictedMolecularWeight":"74.29 kDa","actualMolecularWeight":"74.29 kDa","aminoAcidEnd":698,"aminoAcidStart":25,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P19320","tags":[]}]
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出荷温度及び保存条件

製品の状態
Lyophilized
出荷温度
Ambient - Cannot Ship with Ice
短期保存温度
-20°C
長期保存温度
-20°C
True
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VCAM1 also known as vascular cell adhesion molecule 1 plays an important role in mediating adhesion and signal transduction. VCAM1 has an approximate molecular mass of 110 kDa. It is expressed on the surface of endothelial cells and can be upregulated by cytokines released during inflammation. The protein serves as a ligand for the integrin VLA-4 also known as α4β1 and contributes to the adhesion of leukocytes to endothelial cells.
Biological function summary

Adhesion molecules like VCAM1 participate in immune response by recruiting leukocytes to sites of inflammation. VCAM1 helps guide immune cells to directed locations where they perform defense activities. Although VCAM1 is not part of any known large protein complex its interaction with integrins facilitates the migration of immune cells across the endothelium and into tissue.

Pathways

VCAM1 contributes significantly to the leukocyte extravasation process in inflammatory pathways. It plays a role in both the cytokine-cytokine receptor interaction pathway and the NF-kB signaling pathway which are essential for immune response and cell signaling. Through these pathways it interacts with proteins like ICAM1 and various chemokines helping to coordinate the immune cell movement through vascular tissue barriers.

Abnormal regulation of VCAM1 links to various cardiovascular diseases such as atherosclerosis and inflammatory diseases like rheumatoid arthritis. The protein levels may increase in these conditions promoting excessive immune cell recruitment and tissue damage. VCAM1 also interfaces with other proteins like ICAM1 in these pathological states which together contribute to the progression and severity of these diseases.
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一般的な情報

機能

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).

翻訳後修飾

Cleaved by the metalloproteinase ADAM17 to generate the soluble form.. Sialoglycoprotein.. Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination; leading to proteasomal degradation.

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製品プロトコール

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ターゲットの情報

Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed : 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed : 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed : 22970700). Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed : 35210567).
See full target information VCAM1
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