AB105601

Recombinant Human VASP protein (His tag N-Terminus)

Name: Recombinant Human VASP protein (ab105601) | Abcam
Brand: Abcam Limited
SKU: AB105601
Price: 115000 JPY
Availability: InStock

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(2 Publications)

Recombinant Human VASP protein (His tag N-Terminus) is a Human Fragment protein, in the 1 to 343 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

別名を表示する

Vasodilator-stimulated phosphoprotein, VASP

1 Images

SDS-PAGE - Recombinant Human VASP protein (His tag N-Terminus) (AB105601)

SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human VASP protein (His tag N-Terminus) (AB105601)

15% SDS-PAGE showing ab105601 (3 μg) at approximately 37.5 kDa.

Key facts

精製度

>85% SDS-PAGE

ab105601 is purified using conventional chromatography techniques.

発現系

Escherichia coli

アプリケーション

Mass Spec, SDS-PAGE

applications

生物活性

アクセッション番号

P50552

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"linker":null,"sequence":"MGSSHHHHHHSSGLVPRGSHMSETVICSSRATVMLYDDGNKRWLPAGTGPQAFSRVQIYHNPTANSFRVVGRKMQPDQQVVINCAIVRGVKYNQATPNFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALEGGGPPPPPALPTWSVPNGPSPEEVEQQKRQQPGPSEHIERRVSNAGGPPAPPAGGPPPPPGPPPPPGPPPPPGLPPSGVPAAAHGAGGGPPPAPPLPAAQGPGGGGAGAPGLAAAIAGAKLRKVSKQEEASGGPTAPKAESGRSGGGGLMEEMNAMLARRRKATQVGEKTPKDESANQEEPEARVPAQSESVRRPWEKNSTTLPRMKSSSSVTTSETQPCTPSSSDYSD","proteinLength":"Fragment","predictedMolecularWeight":"37.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":343,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P50552","tags":[{"tag":"His","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

製品の状態

Liquid

出荷温度

Blue Ice

短期保存温度

-20°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

False

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

VASP also known as Vasodilator-Stimulated Phosphoprotein is a 46-50 kDa protein involved in actin filament assembly and elongation. It plays an important role in cellular motility and the maintenance of cell shape. VASP is expressed in many cell types but is notably present in platelets and endothelial cells. It is an important player in signal transduction pathways that lead to cytoskeletal reorganization influencing cell movement and adhesion dynamics.

Biological function summary

VASP is an important component in cell movement regulation. It acts within a larger complex of actin-binding proteins that control the assembly of actin filaments. This protein influences cell adhesion migration and signal transduction processes. Through these actions VASP influences cellular responses to environmental cues making it essential for cellular dynamics and integrity during development and wound healing.

Pathways

VASP connects to significant signaling pathways such as the PI3K/Akt pathway and the cAMP pathway. It often interacts with proteins like c-Abl tyrosine kinase and Mena contributing to actin dynamics regulation. These pathways are critical for transducing signals from extracellular environments to intracellular responses allowing for coordinated cellular functions in processes such as cell migration and growth.

VASP plays a role in cardiovascular disease and cancer-related metastasis due to its function in cell motility and adhesion. Abnormal VASP activity is associated with impaired endothelial cell function and contributes to atherosclerosis development. Additionally it interacts with proteins like Mena and fascin in cancer cells aiding in metastatic progression by enhancing motility and invasive capabilities.

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一般的な情報

機能

Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation.

配列の類似性

Belongs to the Ena/VASP family.

翻訳後修飾

Major substrate for cAMP-dependent (PKA) and cGMP-dependent protein kinase (PKG) in platelets. The preferred site for PKA is Ser-157, the preferred site for PKG/PRKG1, Ser-239. In ADP-activated platelets, phosphorylation by PKA or PKG on Ser-157 leads to fibrinogen receptor inhibition. Phosphorylation on Thr-278 requires prior phosphorylation on Ser-157 and Ser-239. In response to phorbol ester (PMA) stimulation, phosphorylated by PKC/PRKCA. In response to thrombin, phosphorylated by both PKC and ROCK1. Phosphorylation at Thr-278 by AMPK does not require prior phosphorylation at Ser-157 or Ser-239. Phosphorylation at Ser-157 by PKA is required for localization to the tight junctions in epithelial cells. Phosphorylation modulates F-actin binding, actin filament elongation and platelet activation. Phosphorylation at Ser-322 by AMPK also alters actin filament binding. Carbon monoxide (CO) promotes phosphorylation at Ser-157, while nitric oxide (NO) promotes phosphorylation at Ser-157, but also at Ser-239. Response to NO and CO is blunted in platelets from diabetic patients, and VASP is not phosphorylated efficiently at Ser-157 and Ser-239.

細胞内局在性

Cytoskeleton

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information VASP

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e37110 PubMed39296110

2024

Anti-vasodilator-stimulated phosphoprotein (VASP) antibodies are associated with neuropsychiatric disorders in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Zhu,Yan Liu,Jiayi Xu,Hang Yang,Yi Zhao,Yi Liu

Communications biology 5:795 PubMed35941177

2022

Cooperation of Striatin 3 and MAP4K4 promotes growth and tissue invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica Migliavacca,Buket Züllig,Charles Capdeville,Michael A Grotzer,Martin Baumgartner

View all publications

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Recombinant Human VASP protein (His tag N-Terminus)