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AB49001

Recombinant Human VAMP2 protein (His tag N-Terminus)

Recombinant Human VAMP2 protein (His tag N-Terminus)

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Recombinant Human VAMP2 protein (His tag N-Terminus) is a Human Fragment protein, in the 1 to 89 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE.

別名を表示する

SYB2, VAMP2, Vesicle-associated membrane protein 2, VAMP-2, Synaptobrevin-2

1 Images
SDS-PAGE - Recombinant Human VAMP2 protein (His tag N-Terminus) (AB49001)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human VAMP2 protein (His tag N-Terminus) (AB49001)

ab49001 run on a 15% SDS-PAGE gel with molecular weight markers.

Key facts

精製度

>95% SDS-PAGE

発現系

Escherichia coli

タグ

His tag N-Terminus

アプリケーション

SDS-PAGE

applications

生物活性

No

アクセッション番号

P63027

アニマルフリー

No

キャリアフリー

No

種

Human

バッファー組成

pH: 7.4 Constituents: PBS, 0.0292% EDTA

storage-buffer

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組合せ製品

Reagents, Controls & Accessories

AB234592

Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa)

SDS-PAGE - Prestained Protein Ladder - Extra broad molecular weight (6.5 - 270 kDa) (AB234592)

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  • 0
Proteins & Peptides

AB74529

Recombinant Human SNAP25 protein (Tag Free)

Western blot - Recombinant Human SNAP25 protein (Tag Free) (AB74529)

  • 0
  • 0
Primary Antibodies

AB181869

Anti-VAMP2 antibody [EPR12790]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] (AB181869)

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  • 1
Proteins & Peptides

AB316480

Recombinant Syntaxin 1a Protein Standard (His tag)

Sandwich ELISA - Recombinant Syntaxin 1a Protein Standard (His tag) (AB316480)

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  • 0
Proteins & Peptides

AB288788

Recombinant Human WIF1 protein

HPLC - Recombinant Human WIF1 protein (AB288788)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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配列情報

[{"linker":null,"sequence":"MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSHMSATAATAPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYW","proteinLength":"Fragment","predictedMolecularWeight":"13.8 kDa","actualMolecularWeight":null,"aminoAcidEnd":89,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P63027","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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出荷温度及び保存条件

製品の状態
Liquid
出荷温度
Blue Ice
短期保存温度
-20°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
False
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Vesicle-associated membrane protein 2 (VAMP2) also known as synaptobrevin 2 is a member of the SNARE protein family with a molecular mass of approximately 13 kDa. VAMP2 plays an important role in vesicle trafficking and membrane fusion particularly in the nervous system. It is prominently expressed in the brain where it facilitates neurotransmitter release by forming SNARE complexes essential for synaptic vesicle fusion with the presynaptic membrane.
Biological function summary

VAMP2 interacts with other SNARE proteins such as synaptosomal-associated protein 25 (SNAP-25) and syntaxin-1 to form stable complexes necessary for synaptic vesicle docking and exocytosis. As part of the SNARE complex VAMP2 contributes to the precise regulation of neurotransmitter release in neurons which is critical for synaptic transmission and plasticity. Its function is vital for the communication between neurons and proper brain function.

Pathways

VAMP2 plays a significant role in the synaptic vesicle cycle and neurotransmitter release pathways. It is central to the exocytosis pathway where it interacts with Rab3a a small GTPase that regulates vesicle docking. By participating in these pathways VAMP2 ensures efficient neurotransmission and contributes to neuronal communication processes. VAMP2's interaction with related proteins like SNAP-25 and syntaxin-1 highlights its importance in these synaptic processes.

VAMP2 has associations with conditions such as epilepsy and neurodegenerative diseases. Alterations in VAMP2 expression or function can disrupt normal neurotransmitter release impacting synaptic function and leading to neurological symptoms. VAMP2's relationship with SNAP-25 and syntaxin-1 further implicates it in these disorders as these proteins collectively regulate neural communication. Understanding VAMP2's role in these illnesses aids in the development of potential therapeutic strategies targeting SNARE complex dysfunctions.
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一般的な情報

機能

Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles (By similarity) (PubMed : 30929742). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.

配列の類似性

Belongs to the synaptobrevin family.

翻訳後修飾

Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2.. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type B (BoNT/B, botB) which hydrolyzes the 76-Gln-|-Phe-77 bond and probably inhibits neurotransmitter release (PubMed:7803399).. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type D (BoNT/D, botD) which probably hydrolyzes the 59-Lys-|-Leu-60 bond and inhibits neurotransmitter release (PubMed:22289120). Note that humans are not known to be infected by C.botulinum type D.. (Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type F (BoNT/F, botF) which hydrolyzes the 58-Gln-|-Lys-59 bond and probably inhibits neurotransmitter release (PubMed:19543288).. (Microbial infection) Targeted and hydrolyzed by C.tetani tetanus toxin (tetX) which hydrolyzes the 76-Gln-|-Phe-77 bond and probably inhibits neurotransmitter release (PubMed:7803399).

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製品プロトコール

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ターゲットの情報

Involved in the targeting and/or fusion of transport vesicles to their target membrane (By similarity). Major SNARE protein of synaptic vesicles which mediates fusion of synaptic vesicles to release neurotransmitters. Essential for fast vesicular exocytosis and activity-dependent neurotransmitter release as well as fast endocytosis that mediates rapid reuse of synaptic vesicles (By similarity) (PubMed : 30929742). Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.
See full target information VAMP2
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