AB269117

Recombinant ヒト USP16 protein (Active) (GST tag N-Terminus)

Recombinant human USP16 protein (Active) (GST tag N-Terminus)

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Recombinant human USP16 protein (Active) (GST tag N-Terminus) is a Human Fragment protein, in the 187 to 823 aa range, expressed in Baculovirus infected Sf9 cells, with >85%, suitable for SDS-PAGE, FuncS.

別名を表示する

MSTP039, USP16, Ubiquitin carboxyl-terminal hydrolase 16, Deubiquitinating enzyme 16, Ubiquitin thioesterase 16, Ubiquitin-processing protease UBP-M, Ubiquitin-specific-processing protease 16

2 Images

Functional Studies - Recombinant human USP16 protein (Active) (GST tag N-Terminus) (AB269117)

FuncS

Supplier Data

Functional Studies - Recombinant human USP16 protein (Active) (GST tag N-Terminus) (AB269117)

The specific activity of ab269117 was 24 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.

SDS-PAGE - Recombinant human USP16 protein (Active) (GST tag N-Terminus) (AB269117)

SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human USP16 protein (Active) (GST tag N-Terminus) (AB269117)

Recombinant human USP16 protein (ab269117) resolved on a SDS-PAGE gel.

Key facts

精製度

>85% SDS-PAGE

Affinity purified.

発現系

Baculovirus infected Sf9 cells

アプリケーション

FuncS, SDS-PAGE

applications

生物活性

Yes

生物学的活性

The specific activity of ab269117 was 24 nmol/min/mg in a DUB assay using ubiquitin-based proluciferin substrate.

アクセッション番号

Q9Y5T5

アニマルフリー

キャリアフリー

種

Human

バッファー組成

pH: 7.5 Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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配列情報

[{"linker":null,"sequence":"MNSPCQITVKGLSNLGNTCFFNAVMQNLSQTPVLRELLKEVKMSGTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNEMQETKKGVVTPKELFSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEELKNKVKDYEKKKSMPSFVDRIFGGELTSMIMCDQCRTVSLVHESFLDLSLPVLDDQSGKKSVNDKNLKKTVEDEDQDSEEEKDNDSYIKERSDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQGKVLHLNDICTIDHPEDSEYEAEMSLQGEVNIKSNHISQEGVMHKEYCVNQKDLNGQAKMIESVTDNQKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAYLTEGSNGEVDISNGFKNLNLNAALHPDEINIEILNDSHTPGTKVYEVVNEDPETAFCTLANREVFNTDECSIQHCLYQFTRNEKLRDANKLLCEVCTRRQCNGPKANIKGERKHVYTNAKKQMLISLAPPVLTLHLKRFQQAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEENTRVLYSLYGVVEHSGTMRSGHYTAYAKARTANSHLSNLVLHGDIPQDFEMESKGQWFHISDTHVQAVPTTKVLNSQAYLLFYERIL","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":823,"aminoAcidStart":187,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q9Y5T5","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification

出荷温度

Dry Ice

短期保存温度

-80°C

長期保存温度

-80°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

True

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

USP16 also known as Ubiquitin Specific Peptidase 16 functions as a deubiquitinating enzyme that regulates protein degradation by cleaving ubiquitin from ubiquitin-conjugated protein substrates. It has a molecular mass of approximately 97 kDa. USP16 is expressed in various tissues with high levels in the brain and heart. It has an integral role in maintaining protein homeostasis by regulating degradation pathways necessary for cellular function.

Biological function summary

The enzymatic activity of USP16 removes ubiquitin moieties contributing to the stabilization of target proteins. It participates in several cellular processes including cell cycle regulation DNA damage response and chromatin remodeling. USP16 is associated with the Polycomb group (PcG) complex which plays a role in transcriptional repression. This involvement in epigenetic regulation highlights its importance in gene expression and cellular differentiation.

Pathways

USP16 is significant in the Wnt signaling pathway and the Hedgehog signaling pathway. In the Wnt pathway USP16 regulates beta-catenin degradation influencing cell proliferation and differentiation. Through the Hedgehog pathway USP16 impacts the stability of GLI transcription factors affecting developmental processes and tissue patterning. USP16 interacts with proteins like Axin and APC in the Wnt pathway illustrating its role in complex regulatory networks.

Researchers have found connections between USP16 dysregulation and cancer particularly leukemia and neurodegenerative diseases like Alzheimer's disease. In cancer USP16 overexpression can lead to increased cell proliferation through pathways involving proteins such as MYC and Cyclin D1. Related to neurodegenerative disorders USP16's interaction with proteins like amyloid precursor protein (APP) contributes to pathologies like Alzheimer's where proteostasis and ubiquitination play critical roles. Understanding USP16's functions and connections can provide insights into therapeutic targets for these diseases.

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一般的な情報

機能

Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator (PubMed : 17914355). Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis (PubMed : 17914355). In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination (PubMed : 17914355). Prefers nucleosomal substrates (PubMed : 17914355). Does not deubiquitinate histone H2B (PubMed : 17914355). Also deubiquitinates non-histone proteins, such as ribosomal protein RPS27A : deubiquitination of monoubiquitinated RPS27A promotes maturation of the 40S ribosomal subunit (PubMed : 32129764). Also mediates deubiquitination of tektin proteins (TEKT1, TEKT2, TEK3, TEKT4 and TEKT5), promoting their stability.

配列の類似性

Belongs to the peptidase C19 family. USP16 subfamily.

翻訳後修飾

Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. Phosphorylation by AURKB enhances the deubiquitinase activity.

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製品プロトコール

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

See full target information USP16

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Abcam product promise

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Recombinant ヒト USP16 protein (Active) (GST tag N-Terminus)

Recombinant human USP16 protein (Active) (GST tag N-Terminus)

Functional Studies - Recombinant human USP16 protein (Active) (GST tag N-Terminus) (AB269117)

SDS-PAGE - Recombinant human USP16 protein (Active) (GST tag N-Terminus) (AB269117)

Key facts

精製度

発現系

タグ

アプリケーション

生物活性

生物学的活性

アクセッション番号

アニマルフリー

キャリアフリー

種

バッファー組成

組合せ製品

Primary Antibodies

Primary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

配列情報

出荷温度及び保存条件

製品の状態

精製方法

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

一般的な情報

機能

配列の類似性

翻訳後修飾

製品プロトコール

ターゲットの情報

Abcam product promise